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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

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Related Experiment Video

Updated: May 8, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
06:15

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

Immunoassay techniques.

Michael J Wheeler1

  • 1Consultant Clinical Scientist, The Old Dairy , Exmouth, UK.

Methods in Molecular Biology (Clifton, N.J.)
|September 3, 2013
PubMed
Summary
This summary is machine-generated.

Immunoassay revolutionized hormone measurement, offering practical methods for producing reagents and performing assays for various hormones. This guide provides foundational techniques for developing reliable immunoassays quickly.

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Last Updated: May 8, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
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Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Published on: December 12, 2011

Area of Science:

  • Biochemistry
  • Immunology
  • Analytical Chemistry

Background:

  • Immunoassay represents a significant advancement in hormone measurement technology.
  • Commercial availability of reagents and kits is limited for novel or less common hormones.

Purpose of the Study:

  • To explain the fundamental principles of immunoassay reactions.
  • To provide practical methods for developing immunoassays and immunometric assays.
  • To guide the production of reagents for both antigenic and hapten hormones.

Main Methods:

  • Detailed explanation of the basic immunoassay reaction.
  • Simple methods for conducting immunoassays and immunometric assays.
  • Procedures for preparing labeled hormones and hormone reagents.
  • Various separation techniques for immunoassay procedures.

Main Results:

  • Established methods for producing usable immunoassays in a short timeframe.
  • Demonstrated applicability across a wide range of hormone assays.
  • Validated techniques that have proven reliable over time.

Conclusions:

  • Immunoassay techniques are essential for accurate hormone level determination.
  • The described methods empower researchers to develop custom immunoassays for diverse hormonal analytes.
  • This work facilitates the advancement of endocrinology and related fields through accessible assay development.