Related Experiment Video
Updated: May 8, 2026

A Novel Method: Super-selective Adrenal Venous Sampling
Published on: September 15, 2017
Measurement of aldosterone in blood
1Imperial College Healthcare NHS Trust, Charing Cross Hospital, London, UK.
Abstract:
This chapter describes the measurement of aldosterone concentration in plasma or serum. Aldosterone specificity is improved by first extracting the aldosterone into dichloromethane. A radioimmunoassay is then performed on the dried and reconstituted extract using an anti-aldosterone antibody and iodinated aldosterone. The antibody-bound fraction is separated from the unbound fraction using activated charcoal. The notes section provides extra information for steps in the assay that can be problematic.
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