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Updated: May 8, 2026

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization
Published on: January 24, 2025
Using protein nanofibrils to remove azo dyes from aqueous solution by the coagulation process.
Dina Morshedi1, Zeinab Mohammadi, Masoud Mashhadi Akbar Boojar
1Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Proteinaceous nanofibrils effectively decolorize hazardous azo dyes in wastewater, showing higher efficiency than charcoal. These eco-friendly coagulants offer a promising solution for colored wastewater treatment without cytotoxicity.
Area of Science:
- Environmental Science
- Materials Science
- Biotechnology
Background:
- Industrial azo dyes pose significant environmental challenges due to widespread application.
- Effective remediation strategies for azo dye-contaminated water are urgently needed.
Purpose of the Study:
- To evaluate proteinaceous nanofibrils as coagulants for azo dye decolorization.
- To investigate the mechanism and efficiency of dye removal by nanofibrils.
Main Methods:
- Tested nanofibrils for decolorizing five types of azo dyes (Acid Red 88, Bismarck Brown R, Direct Violet 51, Reactive Black 5, Congo Red).
- Assessed the impact of temperature, ionic strength, and pH on dye removal efficiency and coagulation time.
- Compared nanofibril performance with charcoal for azo dye remediation.
Main Results:
- Proteinaceous nanofibrils demonstrated variable but high dye removal efficiency (60-98%), dependent on dye structure and solution conditions.
- Increased temperature and ionic strength accelerated dye removal.
- Nanofibrils outperformed charcoal in dye removal efficiency and showed no detected cytotoxicity in treated solutions.
Conclusions:
- Proteinaceous nanofibrils are effective and safe coagulants for treating azo dye wastewater.
- Their ease of production, dispersion, and lack of harmful by-products make them advantageous.
- This approach integrates biological and physicochemical processes for sustainable wastewater treatment.
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Aryldiazonium Salts to Azo Dyes: Diazo Coupling
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