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Plasma and tissue-free amino acids in the developing chick
1Laboratoire de Physiologie Métabolique et Nutrition, Université Pierre-et-Marie-Curie, Paris, France.
Annals of Nutrition & Metabolism
|January 1, 1990
Summary
This study analyzed free and bound amino acids in young fasted chicks. Tissue amino acid composition stabilizes early, unlike plasma, which changes with age.
Area of Science:
- Biochemistry
- Animal Nutrition
- Physiology
Background:
- Amino acids are crucial for metabolic processes in animals.
- Understanding amino acid dynamics is vital for optimizing poultry growth and health.
- Fasting impacts nutrient availability and metabolic pathways.
Purpose of the Study:
- To investigate the relationship between free and protein-bound amino acids in chick tissues and plasma.
- To determine how fasting affects amino acid profiles in different tissues (pectoral muscle, liver, brain) and plasma.
- To examine the developmental changes in amino acid composition during early chick growth.
Main Methods:
- Analysis of free amino acids in plasma and tissue cytosolic S105 supernatant using High-Performance Liquid Chromatography (HPLC).
- Deproteinization of samples via ultrafiltration prior to HPLC analysis.
- Parallel analysis of protein-bound amino acids in tissues.
Main Results:
- A clear relationship was observed between free and bound amino acid pools within tissues.
- Gluconeogenic amino acids showed a strong correlation between plasma and pectoral muscle free amino acid levels.
- Free amino acid composition in tissues reached a stable state early in development.
- Plasma free amino acid composition exhibited significant age-related changes.
Conclusions:
- Tissue amino acid homeostasis is established early in young chicks.
- Plasma amino acid profiles are more dynamic and sensitive to age during early development.
- Specific amino acid relationships (gluconeogenic) exist between circulation and muscle tissue.