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Updated: May 8, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

Hyperspectral fluorescence lifetime imaging for optical biopsy.

Zhaojun Nie¹, Ran An, Joseph E Hayward

¹McMaster University, School of Biomedical Engineering, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada.

Journal of Biomedical Optics

|September 5, 2013

Summary

A new hyperspectral fluorescence lifetime imaging (FLIM) instrument offers optical biopsy capabilities. This advanced tool provides multidimensional tissue analysis for improved clinical diagnosis.

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Area of Science:

Biomedical Optics
Medical Imaging
Spectroscopy

Background:

Accurate tissue diagnosis is crucial for effective clinical decision-making.
Current optical biopsy tools often lack comprehensive spectral and temporal resolution.
Endogenous fluorophores in tissue offer valuable diagnostic information.

Purpose of the Study:

To develop and validate a hyperspectral fluorescence lifetime imaging (FLIM) instrument.
To enable spectrally, temporally, and spatially resolved analysis of endogenous fluorophores in biological tissue.
To establish a novel optical biopsy tool for enhanced clinical tissue diagnosis.

Main Methods:

Development of a hyperspectral FLIM instrument utilizing an acousto-optic tunable filter (AOTF) for rapid wavelength switching.

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Last Updated: May 8, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications

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Real-time collection of time-resolved fluorescence decay using a photomultiplier tube and high-speed digitizer.

Performance characterization and validation using fluorescence tissue phantoms and porcine skin specimens.

Main Results:

Demonstration of an instrument capable of spectrally, temporally, and spatially resolving fluorescence signals.
Validation of the dual-arm AOTF design for high spectral throughput and microsecond random wavelength switching.
Successful characterization on tissue phantoms and porcine skin, showing potential for clinical application.

Conclusions:

The developed hyperspectral FLIM instrument serves as a promising optical biopsy tool.
Multidimensional fluorescence information aids in more accurate clinical tissue diagnosis.
The instrument's rapid wavelength switching and high spectral resolution are advantageous for time-critical medical applications.