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Quantification of Circular RNAs Using Digital Droplet PCR
08:39

Quantification of Circular RNAs Using Digital Droplet PCR

Published on: September 16, 2022

Tolerance of droplet-digital PCR vs real-time quantitative PCR to inhibitory substances

Tanis C Dingle1, Ruth Hall Sedlak, Linda Cook

  • 1Department of Laboratory Medicine University of Washington Seattle, WA.

Clinical Chemistry
|September 5, 2013
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...