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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Updated: May 8, 2026

Bimolecular Fluorescence Complementation
08:54

Bimolecular Fluorescence Complementation

Published on: April 15, 2011

Simultaneous visualization of multiple protein interactions using multicolor bimolecular fluorescence complementation

Tom K Kerppola

    Cold Spring Harbor Protocols
    |September 5, 2013
    PubMed
    Summary

    This study presents a multicolor bimolecular fluorescence complementation method for analyzing protein interactions. The technique is adaptable for various cell types and organisms, enabling broad application in biological research.

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    Last Updated: May 8, 2026

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    Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction
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    Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves

    Published on: March 9, 2014

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Cell Biology

    Background:

    • Protein-protein interactions are crucial for cellular functions.
    • Analyzing these interactions is vital for understanding biological processes.
    • Existing methods may have limitations in versatility or scope.

    Purpose of the Study:

    • To develop a multicolor bimolecular fluorescence complementation (BiFC) assay.
    • To enable the analysis of alternative protein interactions.
    • To create a versatile tool applicable across different biological systems.

    Main Methods:

    • Utilizing bimolecular fluorescence complementation (BiFC).
    • Employing a multicolor detection strategy.
    • Genetically modifying cells or organisms to express fusion proteins.

    Main Results:

    • Demonstrated the procedure's effectiveness in cultured mammalian cells.
    • Showcased the adaptability of the multicolor BiFC technique.
    • Confirmed its potential for analyzing diverse protein interaction networks.

    Conclusions:

    • The multicolor BiFC procedure is a powerful tool for studying protein interactions.
    • Its adaptability allows for application in various cell types and aerobic organisms.
    • This method enhances the study of molecular mechanisms through protein interaction analysis.