Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Muscles of the Leg that Move the Foot and Toes01:28

Muscles of the Leg that Move the Foot and Toes

The human leg comprises an intricate system of muscles that facilitate the movement of feet and toes. Within this system, the muscles are categorized into the anterior, lateral, and posterior compartments, each with a unique set of muscles carrying out specific functions.
Anterior Compartment
The anterior compartment includes muscles that contribute to the dorsiflexion of the foot. This compartment houses the tibialis anterior, extensor hallucis longus, and extensor digitorum longus muscles.
Imprinting01:22

Imprinting

Behavioral imprinting is observed in some newborn animals and occurs when they develop strong and specific attachments to another animal (usually a parent) following brief, early-life exposures. Offspring imprint onto parents within a brief period after birth or hatching; this time window is called the critical period. Once imprinting occurs, the bond established between the parents and their offspring is usually long-lasting.
Lampbrush Chromosomes01:51

Lampbrush Chromosomes

In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops resemble the...
Tip-of-the-Tongue Phenomenon01:10

Tip-of-the-Tongue Phenomenon

The tip-of-the-tongue (TOT) phenomenon is a cognitive experience characterized by a temporary inability to retrieve specific information from memory despite having a strong feeling of knowing the information. Although individuals cannot access the target word or detail, they frequently recall related elements, such as its initial letter, syllable count, or context. This partial retrieval often causes frustration, as one might recognize a familiar face or know that a name starts with a specific...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Kaposi's Sarcoma-Associated Herpesvirus Utilizes and Manipulates RNA N<sup>6</sup>-Adenosine Methylation To Promote Lytic Replication.

Journal of virology·2017
Same author

NOTE FROM THE EDITOR.

RNA (New York, N.Y.)·2016
Same author

Erratum: Preparation of Nuclear Extracts from HeLa Cells.

Cold Spring Harbor protocols·2016
Same author

Cotranslational microRNA mediated messenger RNA destabilization.

eLife·2016
Same author

Preparing Cellular DNA from Nuclei or Whole Cells.

Cold Spring Harbor protocols·2015
Same author

Removal of rRNA from Deproteinized, Phenol-Extracted Total RNA by Hybrid Selection.

Cold Spring Harbor protocols·2015
Same journal

Emerging Trends in Mass Spectrometry-Based Quantitative Proteome and Phosphoproteome Profiling in Maize.

Cold Spring Harbor protocols·2026
Same journal

Sample Preparation for Quantitative Proteome and Phosphoproteome Profiling of Maize Tissues.

Cold Spring Harbor protocols·2026
Same journal

High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic <i>E. coli</i>.

Cold Spring Harbor protocols·2025
Same journal

Grain Quality in Maize.

Cold Spring Harbor protocols·2025
Same journal

High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples.

Cold Spring Harbor protocols·2025
Same journal

Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource.

Cold Spring Harbor protocols·2025
See all related articles

Related Experiment Video

Updated: May 8, 2026

Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs
10:37

Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs

Published on: May 10, 2018

Toeprinting.

Timothy W Nilsen

    Cold Spring Harbor Protocols
    |September 5, 2013
    PubMed
    Summary
    This summary is machine-generated.

    Toeprinting precisely maps ribosome positions on messenger RNAs (mRNAs). This molecular biology technique uses reverse transcriptase enzyme activity to identify the location of protein complexes on RNA molecules.

    More Related Videos

    Spotting Cheetahs: Identifying Individuals by Their Footprints
    09:47

    Spotting Cheetahs: Identifying Individuals by Their Footprints

    Published on: May 1, 2016

    Low-Cost Gait Analysis for Behavioral Phenotyping of Mouse Models of Neuromuscular Disease
    05:53

    Low-Cost Gait Analysis for Behavioral Phenotyping of Mouse Models of Neuromuscular Disease

    Published on: July 18, 2019

    Related Experiment Videos

    Last Updated: May 8, 2026

    Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs
    10:37

    Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs

    Published on: May 10, 2018

    Spotting Cheetahs: Identifying Individuals by Their Footprints
    09:47

    Spotting Cheetahs: Identifying Individuals by Their Footprints

    Published on: May 1, 2016

    Low-Cost Gait Analysis for Behavioral Phenotyping of Mouse Models of Neuromuscular Disease
    05:53

    Low-Cost Gait Analysis for Behavioral Phenotyping of Mouse Models of Neuromuscular Disease

    Published on: July 18, 2019

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biochemistry

    Background:

    • Toeprinting is a molecular biology technique used to determine the precise location of ribosomes on messenger RNA (mRNA).
    • The method relies on the reverse transcriptase enzyme pausing when encountering a blocking complex on the RNA strand.

    Purpose of the Study:

    • To describe a specific toeprinting procedure developed for mapping the exon junction complex on spliced mRNAs.
    • To highlight the versatility of toeprinting beyond ribosome profiling, applicable to various RNA-binding complexes.

    Main Methods:

    • Utilizes reverse transcriptase to perform primer extension on an RNA template.
    • The length of the extended product is compared to a full-length product to pinpoint the 3' boundary of a bound complex.
    • Adaptation of the technique to detect the exon junction complex, a marker of mRNA splicing.

    Main Results:

    • Successfully adapted toeprinting to identify the position and presence of the exon junction complex on mRNAs.
    • Demonstrates the utility of this method for studying RNA-protein interactions and RNA processing events.

    Conclusions:

    • Toeprinting is a robust technique for precisely locating RNA-bound complexes.
    • The method is adaptable for studying various biological processes, including mRNA splicing and translation.
    • Applicable to any stably bound RNA complex that can impede reverse transcriptase activity.