[Analysis of tritium-labeled glycine and alanine using 3H-NMR]

A method for analysis of three-component isotopic mixtures of tritium-labelled glycine and alanine in D2O solutions has been developed on the basis of high resolution 3H NMR spectra at 266.8 MHz. Determined were composition of the mixtures in molar per cent, as well as geminal and vicinal coupling constants (2JGly3H,H = -16.4 +/- 0.2 Hz; 2JAla3H,H = -14.0 +/- 0.5 Hz; 3JAla3H,H = 7.6 +/- 0.2 Hz) and isotopic shifts (0.21 +/- 0.001 ppm for glycine; 0.026 +/- 0.001 ppm for alanine).