Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of the β correction factor on the accuracy of speckle contrast imaging measurements.

Biomedical optics express·2026
Same author

Lighting the path: a narrative review of non-molecular intraoperative lung imaging modalities.

Journal of thoracic disease·2026
Same author

Rapid Quantitative Imaging of Heterogeneous Tissue Hemoglobin Dynamics Using Spatial Frequency Domain Imaging.

Journal of vascular research·2026
Same author

Optical and acoustic scattering in cutaneous neurofibromas: Implications for early detection.

The Journal of investigative dermatology·2026
Same author

Comparative Evaluation of R134a and HFO-1234ze Cryogen Spray Cooling Using a Mouse Model With Controllable Epidermal Pigmentation.

Lasers in surgery and medicine·2026
Same author

Passive nuclear transport deviates from Fickian behavior in prostate and breast cell types.

Nucleus (Austin, Tex.)·2026
Same journal

Segmentation-guided photon pooling enables robust single-cell analysis and fast fluorescence lifetime imaging microscopy.

Journal of biomedical optics·2026
Same journal

Method of spatial scanning of modulated laser radiation for outline imaging of interphalangeal joints.

Journal of biomedical optics·2026
Same journal

Multimodal optical imaging for the assessment of the teratogenic effects of ethanol on zebrafish development.

Journal of biomedical optics·2026
Same journal

Fluorescence properties of collagen types I-V: a comprehensive study of spectral and lifetime characteristics.

Journal of biomedical optics·2026
Same journal

Spectral dependence of lipofuscin fluorescence lifetimes revealed by FLIM with a superconducting nanowire single-photon detector.

Journal of biomedical optics·2026
Same journal

Building the future of biophotonics through experiential education and seasonal schools.

Journal of biomedical optics·2026
See all related articles

Related Experiment Video

Updated: May 8, 2026

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
11:34

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

Visible spatial frequency domain imaging with a digital light microprojector.

Alexander J Lin1, Adrien Ponticorvo, Soren D Konecky

  • 1University of California, Beckman Laser Institute and Medical Clinic, Department of Surgery, Irvine, CaliforniabUniversity of California, Department of Biomedical Engineering, Irvine, California.

Journal of Biomedical Optics
|September 6, 2013
PubMed
Summary
This summary is machine-generated.

A new flexible LED and modulation element (FLaME) system enables cost-effective, quantitative tissue optical property mapping. This spatial frequency domain imaging (SFDI) platform accurately measures absorption and scattering in tissue, advancing biomedical research and diagnostics.

More Related Videos

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Related Experiment Videos

Last Updated: May 8, 2026

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
11:34

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Optical Spectroscopy

Background:

  • Quantitative tissue optical property measurement is crucial for clinical diagnostics and pre-clinical research.
  • Existing methods can be costly and lack flexibility.
  • There is a need for accessible, high-performance imaging systems.

Purpose of the Study:

  • To present a novel, cost-effective platform for quantitative tissue spectroscopy and imaging.
  • To demonstrate the capability of the flexible LED and modulation element (FLaME) system for spatial frequency domain imaging (SFDI).
  • To validate the system's accuracy in measuring optical properties like absorption (μa) and reduced scattering (μs') in tissue.

Main Methods:

  • Development of a platform using a light-emitting diode (LED) projector, cameras, and a scaled Monte Carlo model.
  • Implementation of spatial frequency domain imaging (SFDI) for model-based reflectance measurements.
  • Validation using tissue-simulating intralipid phantoms and in vivo mouse brain imaging across visible spectral regions.

Main Results:

  • The FLaME system achieved high accuracy in measuring μa (within 11%) and μs' (within 3%) compared to spectrophotometer values in phantoms.
  • Oxy- and total hemoglobin fits showed a 3% difference compared to spectrophotometry.
  • In vivo optical property maps of a mouse brain were successfully acquired, demonstrating the system's potential.

Conclusions:

  • The FLaME system provides a flexible and accurate method for quantitative tissue optical property mapping.
  • The SFDI technique implemented on this platform is suitable for visible spectral regions.
  • The study highlights the correlation between saline's optical clearing effect and decreased skull scattering (μs').