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Mutagenesis and Analysis of Genetic Mutations in the GC-rich KISS1 Receptor Sequence Identified in Humans with Reproductive Disorders
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Analyzing Myc in cell transformation and evolution.

Markus Hartl1, Klaus Bister

  • 1Center for Chemistry and Biomedicine, Institute of Biochemistry, University of Innsbruck, Innsbruck, Austria.

Methods in Molecular Biology (Clifton, N.J.)
|September 6, 2013
PubMed
Summary
This summary is machine-generated.

The Myc oncogene, a key regulator of cell processes, is frequently deregulated in human cancers. This chapter details methods to study Myc

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Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • The Myc oncogene (v-myc) was first identified in avian retroviruses.
  • The cellular Myc protein (c-Myc) is a crucial transcription factor regulating numerous genes.
  • Deregulation of c-Myc contributes significantly to human cancer development.

Purpose of the Study:

  • To describe experimental systems for assessing the oncogenic potential of Myc alleles.
  • To outline strategies for identifying Myc's transcriptional targets in cell transformation.
  • To present methods for investigating the evolutionary history and ancestral function of Myc.

Main Methods:

  • Utilizing cell systems to monitor and quantify oncogenic potential.
  • Employing experimental strategies to isolate and characterize transcriptional targets.
  • Applying procedures to study the evolutionary origin and ancestral structure-function of Myc.

Main Results:

  • Established methodologies for evaluating Myc's oncogenic activity.
  • Identified key transcriptional targets of Myc involved in cell transformation.
  • Provided insights into the evolutionary trajectory and ancestral roles of Myc.

Conclusions:

  • Myc plays a fundamental role in cellular processes and cancer.
  • The described methods facilitate the study of Myc's function and oncogenic potential.
  • Understanding Myc's evolution offers insights into its role in disease.