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Updated: May 8, 2026
An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation
Published on: January 26, 2024
A portable chemical sensor for histidine based on the strategy of click chemistry
Jin Zhou1, Kefeng Xu, Ping Zhou
1MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.
Abstract:
A novel portable chemical sensor is developed in combination of the personal glucose meters (PGM) with click chemistry for sensitive and selective determination of histidine. Invertase-labeled alkynyl-DNA can be modified onto the surfaces of Streptavidin Magnespheres Paramagnetic Particles (PMPs) through copper(I) catalyzed azide-alkyne cycloaddition (CuAAC) reaction and formed invertase-functionalized PMPs, which can be separated easily. The presence of invertase can convert sucrose to glucose and can be monitored by the PGM easily. The presence of histidine can inhibit the CuAAC, so the read-out signal of PGM decreased. The difference in signals from the PGM before and after addition of histidine has a good linear correlation with the logarithm of the histidine concentrations in the range of 0.01~100 μM with a detection limit of 3.4 nM, which is lower than those of many other chemical sensors. Moreover, the assay of histidine in milk samples is demonstrated with satisfactory results.
