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Related Experiment Video

Updated: May 8, 2026

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

Published on: March 28, 2021

Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis.

Chris Gardiner¹, Yannick J Ferreira, Rebecca A Dragovic

¹Nuffield Department of Obstetrics and Gynaecology, University of Oxford, Level 3, Women's Centre, John Radcliffe Hospital, Oxford, UK.

Journal of Extracellular Vesicles

|September 7, 2013

Summary

Nanoparticle tracking analysis (NTA) can reliably size and count extracellular vesicles (EVs) using a new calibration protocol. This standardized method improves NTA accuracy for EV research.

Keywords:

extracellular vesicles light scattering nanoparticle tracking analysis standardisation

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Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing

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Last Updated: May 8, 2026

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

Published on: March 28, 2021

Improving Reproducibility to Meet Minimal Information for Studies of Extracellular Vesicles 2018 Guidelines in Nanoparticle Tracking Analysis

Improving Reproducibility to Meet Minimal Information for Studies of Extracellular Vesicles 2018 Guidelines in Nanoparticle Tracking Analysis

Published on: November 17, 2021

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing

Published on: October 19, 2014

Area of Science:

Biotechnology
Nanotechnology
Biomedical Engineering

Background:

Nanoparticle tracking analysis (NTA) is a widely used technique for characterizing nanoparticles, including extracellular vesicles (EVs).
Current NTA protocols often lack standardization, leading to variability and imprecision in measurements.
Standardization is crucial for the reliable sizing and enumeration of EVs using NTA.

Purpose of the Study:

To propose and detail a standardized protocol for NTA measurements of extracellular vesicles (EVs).
To introduce silica microspheres as a reliable calibration standard for NTA.
To minimize sources of variability and imprecision in NTA for EV analysis.

Main Methods:

Development of a detailed protocol for NTA analysis of EVs.
Utilization of silica microspheres for calibrating NTA instruments.
Application of the protocol to ensure consistent EV sizing and enumeration.

Main Results:

The proposed protocol, incorporating silica microsphere calibration, enhances the standardization of NTA for EV analysis.
The method effectively reduces variability and imprecision commonly observed in NTA measurements of EVs.
Demonstrated feasibility of accurate and reproducible EV characterization using the standardized NTA approach.

Conclusions:

A standardized NTA protocol with silica microsphere calibration offers a robust solution for accurate EV sizing and enumeration.
This approach addresses the current limitations of NTA standardization in EV research.
The protocol facilitates more reliable and reproducible characterization of extracellular vesicles.