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Updated: May 8, 2026
Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
Published on: September 15, 2017
[Effect of paeonol on LPS-induced rat vascular endothelial cell adhesion reaction]
Jun-Jun Chen1, Min Dai, Peng Chen
1Key Laboratory of Xin'an Medicine, Ministry of Education/Department of Pharmacy, Anhui University of Traditional Chinese Medicine/Key Laboratory of Research and Development for Chinese Traditional Medicine, Hefei 230031, China. chenjunjun8812@sina.com
Objective:
To observe the effect of Paeonol (Pae) on lipopolysaccharide (LPS) induced rat mononuclear cells (MCs) adhesion to vascular endothelial cells (VECs) and provide basis foundation for inflammatary mechanisms of Pae against atherosclerosis.
Methods:
Rat vascular endothelial cells were isolated with tissue predigested adherent method. LPS was used as stimulator to induce VEC injury. Serum containing Pae obtained from healthy rats which were given Pae in intragastric. RP-HPLC method was used for detecting the concentration of Pae in serum. MTT assay was used to determine the protective effect of Pae on injured VECs. Rose Bengal Staining was used to detect the effect of Pae on LPS-induced MCs adhesion to VECs.
Results:
LPS induced rat MCs adhesion to VECs. The effect was the strongest when the concentration was 10 ng/mL and incubated with VECs for 5 h. Pae in concentration of 2. 5,5 and 10 microg/mL and incubated for 24 h could effectively inhibit the adhesion and improve the survival rate of LPS injured VECs significantly.
Conclusion:
LPS can damage VECs. Pae could protect VECs from LPS injury via inhibiting MCs adhesion to VECs and improving the VEC survival rate.
Insights
Paeonol (Pae) effectively inhibits lipopolysaccharide (LPS)-induced mononuclear cell (MC) adhesion to vascular endothelial cells (VECs). This action protects VECs from injury and improves their survival rate, offering a potential therapeutic mechanism against atherosclerosis.
Area of Science:
- Cardiovascular Research
- Pharmacology
- Immunology
Background:
- Atherosclerosis involves inflammatory processes, including the adhesion of mononuclear cells (MCs) to vascular endothelial cells (VECs).
- Lipopolysaccharide (LPS) is a potent stimulator of VEC injury and subsequent inflammatory responses.
- Paeonol (Pae) is a traditional Chinese medicine compound with potential anti-inflammatory properties.
Purpose of the Study:
- To investigate the inhibitory effect of Paeonol (Pae) on lipopolysaccharide (LPS)-induced mononuclear cell (MC) adhesion to vascular endothelial cells (VECs).
- To explore the protective mechanisms of Paeonol against LPS-induced VEC injury.
- To provide a foundation for understanding Paeonol's anti-atherosclerotic inflammatory effects.
Main Methods:
- Isolation of rat vascular endothelial cells (VECs) using tissue predigested adherent method.
- Induction of VEC injury using lipopolysaccharide (LPS) as a stimulant.
- Assessment of Paeonol's effect on VEC survival (MTT assay) and MC-VEC adhesion (Rose Bengal Staining).
- Quantification of Paeonol in serum using RP-HPLC.
Main Results:
- Lipopolysaccharide (LPS) significantly induced mononuclear cell (MC) adhesion to vascular endothelial cells (VECs).
- Paeonol, at concentrations of 2.5, 5, and 10 microg/mL, significantly inhibited MC-VEC adhesion.
- Paeonol treatment also significantly improved the survival rate of LPS-injured VECs.
Conclusions:
- Lipopolysaccharide (LPS) damages vascular endothelial cells (VECs) and promotes mononuclear cell (MC) adhesion.
- Paeonol demonstrates a protective effect on VECs against LPS-induced injury.
- Paeonol's mechanism involves inhibiting MC adhesion to VECs and enhancing VEC survival, suggesting potential therapeutic benefits for atherosclerosis.
