[HPLC and UPLC-MS detection of 5-HMF from rabbit ncurolymph after treated with Cornus officinalis]
Objective:
To detect 5-HMF from rabbit ncurolymph after given different dosage of Cornus officinalis via intragastric administration by HPLC and UPLC-MS.
Methods:
Rabbit ncurolymph was cramped out three days after given low, medium, and high dosage of Cornus officinalis. 5-HMF from rabbit ncurolymph was detected with HPLC and UPLC-MS, respectively.
Results:
5-HMF from rabbit ncurolymph was detected with HPLC method only in rabbits given high dose group. Meanwhile, 5-HMF could be detected with UPLC-MS method in rabbits given medium as well as low dose group.
Conclusion:
These results suggest that 5-HMF can cross the blood brain barrier (BBB) of rabbits and enter the rabbit ncurolymph.
Insights
Cornus officinalis administration allows 5-hydroxymethylfurfural (5-HMF) to enter rabbit neurolymph, crossing the blood-brain barrier (BBB). UPLC-MS detected 5-HMF at lower doses than HPLC, indicating higher sensitivity.
Area of Science:
- Pharmacology
- Neuroscience
- Analytical Chemistry
Background:
- Cornus officinalis is a traditional herbal medicine.
- 5-hydroxymethylfurfural (5-HMF) is a key compound in Cornus officinalis.
- The blood-brain barrier (BBB) restricts the passage of substances into the central nervous system.
Purpose of the Study:
- To investigate the detection of 5-HMF in rabbit neurolymph.
- To determine if 5-HMF from Cornus officinalis can cross the BBB.
- To compare the sensitivity of HPLC and UPLC-MS in detecting 5-HMF in neurolymph.
Main Methods:
- Rabbits were administered low, medium, and high doses of Cornus officinalis intragastrically.
- Rabbit neurolymph was collected three days post-administration.
- 5-HMF levels in neurolymph were analyzed using High-Performance Liquid Chromatography (HPLC) and Ultra-Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS).
Main Results:
- 5-HMF was detected by HPLC only in rabbits receiving the high dose of Cornus officinalis.
- 5-HMF was detected by UPLC-MS in rabbits receiving medium and low doses of Cornus officinalis.
- UPLC-MS demonstrated higher sensitivity in detecting 5-HMF in rabbit neurolymph compared to HPLC.
Conclusions:
- 5-HMF can penetrate the rabbit blood-brain barrier (BBB).
- Cornus officinalis derived 5-HMF enters the rabbit neurolymph.
- UPLC-MS is a more sensitive method for detecting 5-HMF in neurolymph at lower concentrations.
