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Plasmids

Plasmids

Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...

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Related Experiment Video

Updated: May 8, 2026

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Published on: August 27, 2014

Plasmid DNA hydrogels for biomedical applications.

Diana Costa¹, Artur J M Valente², M Graça Miguel²

¹CICS - Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, 6201-001 Covilhã, Portugal.

Advances in Colloid and Interface Science

|September 10, 2013

Summary

This summary is machine-generated.

Researchers developed plasmid DNA (pDNA) microgels for combined gene and drug cancer therapy. These pDNA hydrogels effectively load and release therapeutic agents, offering a promising approach for improved cancer treatment.

Keywords:

Biocompatibility Co-delivery Drug delivery In-vitro transfection Plasmid DNA microgels pDNA delivery

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Last Updated: May 8, 2026

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Published on: August 27, 2014

Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications

Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications

Published on: February 7, 2021

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

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Published on: August 4, 2017

Area of Science:

Biomedical Engineering
Materials Science
Pharmacology

Background:

Plasmid DNA (pDNA) systems are being developed for therapeutic biomedical applications.
Biocompatible pDNA hydrogels (macro and micro) were synthesized via cross-linking.

Purpose of the Study:

Investigate swelling behavior of pDNA gels in solutions with additives.
Elucidate solute release mechanisms from pDNA hydrogels for drug/gene delivery formulation.
Develop a system for co-delivery of genes and anticancer drugs for synergistic cancer therapy.

Main Methods:

Preparation of macro and micro plasmid DNA gels through cross-linking.
Swelling studies of pDNA gels in aqueous solutions with varying additives.
Characterization of solute release kinetics from pDNA hydrogels.
Development and testing of a p53 encoding pDNA microgel for co-delivery.

Main Results:

pDNA gels exhibited distinct swelling properties in response to different aqueous additives.
Fundamental aspects of solute release mechanisms from pDNA hydrogels were clarified.
A novel p53 encoding pDNA microgel demonstrated capability for loading and releasing both pDNA and doxorubicin.
The developed microgel is suitable for combined gene and chemical therapy applications.

Conclusions:

The study provides crucial insights into pDNA hydrogel behavior for formulating effective pDNA carriers.
The developed p53 pDNA microgel shows significant potential for co-delivery of genetic material and chemotherapy drugs.
This research advances the development of synergistic cancer therapies combining gene and chemical treatments.