[Genotoxic effect of cigarette smoke condensate on human diploid cell 2BS strain]

A higher sister chromatid exchange (SCE) frequency (17.0/cell) was observed in 2BS cells treated with cigarette smoke condensate (CSC) at 100 micrograms/ml, as compared with 6.9/cell of the background (P less than 0.01). Chromosomal aberration was significantly increased from 4% to 36% in cells treated with 10 micrograms/ml CSC (P less than 0.001). Micronuclei rate varied with CSC from 4 to 26%. There was a dose-effect response between SCE frequency, chromosomal aberration, micronuclei rate and the dose of CSC. The cell-cycle of 2BS cells was markedly delayed by CSC.