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Updated: May 8, 2026
Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue
Published on: January 6, 2012
A tumor necrosis factor-α inhibitor reduces the embryotoxic effects of endometriotic peritoneal fluid
Yi-Jen Chen1, Hua-Hsi Wu, Wan-Ting Liau
1Department of Obstetrics and Gynecology, Taipei Veterans General Hospital, Taipei, Taiwan; Division of Obstetrics and Gynecology, Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan.
Objective:
To determine whether a tumor necrosis factor-α (TNF-α) inhibitor can reduce the embryotoxicity of the peritoneal fluid (PF) of women with endometriosis.
Design:
Experimental clinical study.
Setting:
University hospital.
Patient(S):
Twelve women with chocolate cysts and 12 control women without endometriosis.
Intervention(S):
None.
Main Outcome Measure(S):
We collected the PF from patients with chocolate cysts (CH-PF) and patients without endometriosis (N-PF) during laparoscopic surgery. For the in vitro studies, development and apoptosis were evaluated in two-cell stage mouse embryos after incubation with CH-PF and N-PF, with or without a TNF-α inhibitor.
Result(S):
We found that CH-PF significantly decreased the rate of blastocyst development and increased the percentage of apoptotic cells in the embryos. Cytokine assays showed that the concentrations of several cytokines, including TNF-α, were higher in embryos incubated with CH-PF than in those incubated with N-PF. Furthermore, the treatment of embryos with TNF-α retarded development and induced apoptosis. Important, adalimumab, a TNF-α inhibitor, effectively abrogated the embryotoxicity that was induced by CH-PF.
Conclusion(S):
These data collectively highlight the crucial role of TNF-α in CH-PF-induced embryotoxicity and suggest that TNF-α inhibitors may be potential therapeutic agents for treating endometriosis-induced infertility.
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