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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Related Experiment Video

Updated: May 8, 2026

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
12:28

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

Published on: November 10, 2017

Integrated electron microscopy: super-duper resolution.

Jacomine Krijnse Locker1, Sandra L Schmid

  • 1Department of Infectious Disease & Core Facility Electron Microscopy, University of Heidelberg, Heidelberg, Germany.

Plos Biology
|September 10, 2013
PubMed
Summary
This summary is machine-generated.

Electron microscopy (EM) reveals the detailed structure of caveolae, specialized cell membrane domains. Advanced EM techniques offer high-resolution insights into caveolae coat composition and formation, driving a resurgence in the field.

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Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
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Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)
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Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

Published on: September 29, 2014

Related Experiment Videos

Last Updated: May 8, 2026

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
12:28

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

Published on: November 10, 2017

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
11:19

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

Published on: March 20, 2018

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

Published on: September 29, 2014

Area of Science:

  • Cell Biology
  • Microscopy
  • Structural Biology

Background:

  • Cellular membranes are organized into distinct subdomains by protein and lipid assemblies.
  • Caveolae are critical membrane subdomains involved in signaling, endocytosis, and lipid homeostasis.
  • Traditional super-resolution microscopy struggles to resolve underlying cellular structures.

Purpose of the Study:

  • To investigate the high-resolution composition and organization of caveolae.
  • To understand the formation mechanisms of these specialized membrane structures.
  • To highlight the advancements and resurgence of electron microscopy (EM) in cell biology.

Main Methods:

  • Utilizing genetically encoded probes for protein localization at sub-10 nm resolution.
  • Employing advanced electron microscopy (EM) instruments for imaging larger cell volumes.
  • Applying computational methods for three-dimensional reconstruction of cellular structures.

Main Results:

  • High-resolution insights into the composition and organization of the caveolae coat.
  • Detailed understanding of the formation process of caveolae.
  • Demonstration of EM's capability to reveal sub-10 nm protein localization and 3D structures.

Conclusions:

  • Advanced EM techniques, including genetically encoded probes and 3D reconstruction, provide unprecedented insights into caveolae.
  • These combined EM approaches are crucial for understanding the structural basis of complex cellular functions.
  • There is a significant resurgence in the application of EM for detailed cellular structure studies.