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Determination of pK(a) values of a triptolide derivative and its impurities by pressure-assisted capillary
Jian-Ling Wang1, Xue-Jiao Xu, Dong-Ying Chen
1Laboratory of Pharmaceutical Analysis, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zuchongzhi Road, Shanghai 201203, PR China.
Abstract:
A pressure-assisted capillary zone electrophoresis method was developed to determine the pKa values of a triptolide derivative (LLDT-246) and its impurities. The method was performed in an uncoated fused-silica capillary under the electric voltage of 18kV and 50mbar of external pressure applied simultaneously. A series of running electrolyte buffers were used with pH ranging between 2.2 and 10.0 with the constant ionic strength of 0.05M. The values of pKa of LLDT-246 and two impurities were calculated based on the pH dependence of effective mobilities (μeff). The pKa value of LLDT-246 was in good agreement with that of determined by potentiometric titration method.
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