Subpopulations of granulosa cells within the human ovarian follicle

Human follicular cells were separated according to their isopycnic densities. Three populations were isolated and identified in terms of their secretion of progesterone and oestradiol. Cells in the least dense population secreted approximately 60% as much progesterone and 20% as much oestradiol per cell as did cells in the two denser bands. It is proposed that cumulus cells compose the least dense band and that another band may be made up of antral cells.