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Nanofiber orientation and surface functionalization modulate human mesenchymal stem cell behavior in vitro.
Yash M Kolambkar1, Mehmet Bajin, Abigail Wojtowicz
11 Wallace H. Coulter Department of Biomedical Engineering, Parker H. Petit Institute for Bioengineering and Bioscience , Georgia Institute of Technology, Atlanta, Georgia .
Tissue Engineering. Part A
|September 12, 2013
Summary
Nanofiber scaffold orientation and GFOGER peptide coating enhance human mesenchymal stem cell (hMSC) migration and osteogenic differentiation for tissue regeneration applications.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Cell Biology
Background:
- Electrospun nanofiber meshes are advanced scaffolds for tissue regeneration.
- Their structure and composition can be modified to influence cellular responses.
- Understanding these modifications is crucial for optimizing regenerative therapies.
Purpose of the Study:
- To investigate how nanofiber orientation and GFOGER peptide surface functionalization affect human mesenchymal stem cell (hMSC) migration and osteogenic differentiation.
- To differentiate between proliferation-driven and direct migratory effects.
- To assess the combined impact of topographical cues and surface chemistry.
Main Methods:
- Fabrication of aligned and randomly oriented poly (ε-caprolactone) nanofiber meshes via electrospinning.
- Surface functionalization of meshes with a collagen-mimetic GFOGER peptide.
- In vitro assessment of hMSC migration into a cell-free zone.
- Mitomycin C treatment to evaluate the contribution of cell proliferation to migration.
Main Results:
- GFOGER peptide functionalization significantly enhanced hMSC migration, proliferation, and osteogenic differentiation.
- Aligned nanofiber meshes promoted cell migration along the fiber direction compared to random meshes.
- Fiber alignment did not influence osteogenic differentiation.
- GFOGER coating increased proliferation-driven migration, while fiber orientation enhanced direct migration.
Conclusions:
- Nanofiber scaffold design, through peptide functionalization and topographical alignment, can effectively modulate hMSC behavior.
- These findings offer strategies for directing cellular responses in tissue engineering scaffolds.
- The study highlights the potential of tailored nanofiber meshes for advancing regenerative medicine.
