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Related Experiment Video

Updated: Mar 24, 2026

Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 BSL-3 Conditions
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Guiding Principles: Reporting Elements for Gastrointestinal Organoid Research.

Kelli L VanDussen1, Tatiana A Karakasheva2, Maxime M Mahe3

  • 1Division of Gastroenterology, Hepatology, and Nutrition, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio; Department of Pediatrics, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio.

Cellular and Molecular Gastroenterology and Hepatology
|March 22, 2026
PubMed
Summary
This summary is machine-generated.

Standardized reporting for gastrointestinal (GI) organoids is crucial. This study provides community-informed guidance and a toolkit to improve reproducibility and data comparison in GI organoid research.

Keywords:
ColonoidEnteroidGastrointestinal organoidInduced pluripotent stem cell derived organoidReporting transparency

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Area of Science:

  • Biomedical research
  • Stem cell biology
  • Gastroenterology

Background:

  • Gastrointestinal (GI) organoids are vital tools for disease modeling, drug discovery, and precision medicine.
  • Increasing publications highlight inconsistent methodological reporting, hindering result interpretation and reproducibility.
  • Lack of standardized reporting impedes progress in GI organoid research.

Purpose of the Study:

  • To establish community-informed guidance for reporting key experimental details in GI organoid research.
  • To enhance transparency, comparability, and reproducibility of GI organoid studies.
  • To provide practical resources for researchers, reviewers, and journals.

Main Methods:

  • Integration of established biomedical reporting frameworks.
  • Inclusion of organoid-focused resources from international organizations.
  • Structured input from the GI organoid community via an expert panel survey.

Main Results:

  • Development of core reporting items for GI organoid studies, including stem cell source, media composition, and culture conditions.
  • Creation of an "Organoid Reporting Toolkit" with checklists and templates.
  • Identification of essential parameters for successful replication of GI organoid experiments.

Conclusions:

  • The proposed guidance and toolkit aim to standardize reporting in GI organoid research.
  • Improved reporting will facilitate cross-study comparison and streamline communication.
  • These resources support methodological innovation while enhancing transparency and reproducibility.