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Updated: May 8, 2026
08:51
Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
Published on: March 15, 2019
Deep sequencing approaches to antibody discovery.
Michael F Naso, Jin Lu, Tadas Panavas1
1Janssen Pharmaceutical, 1400 McKean Road, Spring House, PA 19477, USA. mnaso@its.jnj.com.
Current Drug Discovery Technologies
|September 12, 2013
Summary
Next-generation sequencing, or deep sequencing, revolutionizes antibody discovery by enabling the analysis of millions of molecules. This powerful technology facilitates the identification of unique antibody therapeutics with specific properties and functions.
Area of Science:
- Biotechnology
- Immunology
- Therapeutic Development
Background:
- Antibodies are key therapeutic platforms due to their safety, specificity, and efficacy.
- Traditional antibody discovery methods are limited in analyzing the vast molecular diversity of antibodies.
- Complex targets and the need for unique antibody characteristics pose challenges for conventional discovery.
Purpose of the Study:
- To explore the application of next-generation sequencing (deep sequencing) in antibody discovery.
- To overcome the limitations of traditional antibody generation and analysis systems.
- To enable the discovery of novel antibody therapeutics with enhanced properties.
Main Methods:
- Application of next-generation sequencing (deep sequencing) technologies.
- Analysis of millions of single antibody molecules rapidly.
- Interrogation of antibody diversity for specific characteristics.
Main Results:
- Deep sequencing allows for the analysis of millions of antibody molecules in a short period.
- This technology enables the identification of antibodies with very specific properties and activities.
- It enhances the understanding of species and strain-specific antibody repertoires.
Conclusions:
- Next-generation sequencing significantly expands the scope of antibody discovery.
- This approach allows for the isolation of antibodies with unique and desirable characteristics.
- Deep sequencing is paving the way for the development of next-generation antibody therapeutics.
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