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p27(Kip1) inhibits tissue factor expression

Alexander Breitenstein1, Alexander Akhmedov, Giovanni G Camici

  • 1Cardiology, University Heart Center, University Hospital Zurich, Switzerland; Cardiovascular Research, Physiology Institute, University of Zurich, Switzerland; Center for Integrative Human Physiology (ZHIP), University of Zurich, Switzerland.

Biochemical and Biophysical Research Communications
|September 12, 2013
PubMed
Abstract

Insights

The cyclin-dependent kinase inhibitor p27(Kip1) was found to reduce tissue factor (TF) expression in human aortic endothelial cells. This suggests p27(Kip1) may play a role in inhibiting atherosclerosis and vascular remodeling.

Area of Science:

  • Vascular Biology
  • Cell Cycle Regulation
  • Coagulation Cascade

Background:

  • p27(Kip1) is a cyclin-dependent kinase inhibitor that regulates cell proliferation and is implicated in inhibiting atherosclerosis and vascular remodeling.
  • Tissue factor (TF) initiates the coagulation cascade and its expression is associated with atherosclerosis.
  • The relationship between p27(Kip1) and TF expression has not been previously investigated.

Purpose of the Study:

  • To investigate the effect of p27(Kip1) on tissue factor (TF) expression in human aortic endothelial cells.

Main Methods:

  • Overexpression of p27(Kip1) in human aortic endothelial cells using adenoviral transfection.
  • Quiescence of cells followed by stimulation with TNF-α.
  • Assessment of TF protein expression, activity, mRNA levels, and promoter activity.

Main Results:

  • p27(Kip1) overexpression significantly reduced TF protein expression and activity.
  • p27(Kip1) overexpression decreased cytokine-induced TF mRNA expression.
  • TF promoter activity was reduced by p27(Kip1) overexpression, while MAP kinase activation remained unaffected.

Conclusions:

  • p27(Kip1) inhibits TF expression at the transcriptional level.
  • These findings suggest an interaction between p27(Kip1) and TF in pathological processes like atherosclerosis and vascular remodeling.
Keywords:
AtherosclerosisTissue factorVascular remodelingp27(Kip1)

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