Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 8, 2026

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

Published on: September 7, 2022

Capillary morphogenesis in PEG-collagen hydrogels.

Rahul K Singh¹, Dror Seliktar, Andrew J Putnam

¹Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.

|September 12, 2013

Summary

Researchers developed a new biosynthetic hydrogel for 3D cell culture. This material supports the formation of capillary-like networks, aiding tissue engineering and understanding extracellular matrix (ECM) cues in vascularization.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Combining Supramolecular and Covalent Chemistry to Form Reinforced Fibrillar Network Hydrogels From Fibrinogen Derivatives.

Journal of biomedical materials research. Part A·2026

Same author

Injectable Hydrogel-Based Delivery of Soluble Cripto Protein Enhances Repair After Skeletal Muscle Injury.

ACS biomaterials science & engineering·2026

Same author

A robust and user-agnostic step-emulsion platform for scalable microgel fabrication.

bioRxiv : the preprint server for biology·2026

Same author

Active Microrheology Reveals Distinct ECM Mechanical Signatures Induced by Stromal Cells of Different Tissue Origins during Vascular Morphogenesis.

ACS biomaterials science & engineering·2026

Same author

Amoeboid-mesenchymal transition and the proteolytic control of cancer invasion plasticity.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Amoeboid-Mesenchymal Transition and the Proteolytic Control of Cancer Invasion Plasticity.

bioRxiv : the preprint server for biology·2025

Area of Science:

Biomaterials Science
Tissue Engineering
Cell Biology

Background:

Hydrogels are crucial for 3D cell culture and tissue engineering.
Natural extracellular matrix (ECM) hydrogels support vascularization, but synthetic options are limited.
Understanding how ECM cues regulate vascularization is vital for regenerative medicine.

Purpose of the Study:

To develop a synthetic hydrogel that supports vascularization in vitro.
To independently control physical properties and ligand concentration.
To investigate the role of ECM in capillary morphogenesis.

Main Methods:

Created a biosynthetic hydrogel by conjugating polyethylene glycol diacrylamide with type-I collagen.
Utilized acrylamide crosslinks for independent control of material properties.

Keywords:

Angiogenesis Collagen Endothelial cell Hydrogel Photopolymerization Polyethylene oxide

More Related Videos

Production of Nanofibrillar Patterned Collagen for Tissue Engineering

Production of Nanofibrillar Patterned Collagen for Tissue Engineering

Published on: September 20, 2024

Fabrication of Micropatterned Hydrogels for Neural Culture Systems using Dynamic Mask Projection Photolithography

Fabrication of Micropatterned Hydrogels for Neural Culture Systems using Dynamic Mask Projection Photolithography

Published on: February 11, 2011

Related Experiment Videos

Last Updated: May 8, 2026

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

Published on: September 7, 2022

Production of Nanofibrillar Patterned Collagen for Tissue Engineering

Production of Nanofibrillar Patterned Collagen for Tissue Engineering

Published on: September 20, 2024

Fabrication of Micropatterned Hydrogels for Neural Culture Systems using Dynamic Mask Projection Photolithography

Fabrication of Micropatterned Hydrogels for Neural Culture Systems using Dynamic Mask Projection Photolithography

Published on: February 11, 2011

Photoencapsulated endothelial cells and fibroblasts for co-culture within the hydrogel.

Main Results:

The developed hydrogel demonstrated hydrolytic stability while retaining collagen's enzymatic degradability.
Co-culture of endothelial cells and fibroblasts resulted in capillary vessel-like networks with lumens.
Inhibiting matrix metalloproteinase (MMP) activity prevented capillary formation, mirroring natural ECM processes.

Conclusions:

The biosynthetic hydrogel platform enables deciphering ECM regulation of capillary morphogenesis.
This material supports the formation of vascularized tissue constructs for regenerative medicine applications.
The study highlights the importance of MMP activity in vascularization within engineered tissues.