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Related Concept Videos

Blood Typing01:10

Blood Typing

Understanding an individual's blood group is a critical component of transfusion medicine. It ensures compatibility in blood transfusions, organ transplants, and even during pregnancy. Determining these blood groups involves the ABO and Rh blood typing systems, utilizing specific antigens and corresponding anti-sera to identify an individual's blood type.
Antigens are protein molecules that reside on the surface of red blood cells (RBCs). The ABO and Rh blood typing systems target antigens A,...
Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

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Related Experiment Video

Updated: May 8, 2026

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
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Published on: January 6, 2016

ABO blood-typing using an antibody array technique based on surface plasmon resonance imaging.

Nongluck Houngkamhang1, Apirom Vongsakulyanon, Patjaree Peungthum

  • 1Materials Science and Engineering Programme, Faculty of Science, Mahidol University, Rama 6 Rd., Phayathai, Rajathavee, Bangkok 10400, Thailand. toemsak.sri@mahidol.ac.th.

Sensors (Basel, Switzerland)
|September 12, 2013
PubMed
Summary

Surface plasmon resonance imaging (SPR imaging) offers a rapid method for ABO blood typing using common antibodies. This technique accurately groups blood in under 12 minutes, showing promise for faster diagnostics.

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Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
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Last Updated: May 8, 2026

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
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Published on: January 6, 2016

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
15:27

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

Area of Science:

  • Biomedical Engineering
  • Immunology
  • Analytical Chemistry

Background:

  • Standard ABO blood typing relies on agglutination tests, which can be time-consuming.
  • Developing faster and more efficient blood typing methods is crucial for transfusion medicine.
  • Surface plasmon resonance (SPR) is a label-free optical technique sensitive to binding events.

Purpose of the Study:

  • To evaluate the efficacy of surface plasmon resonance imaging (SPR imaging) for ABO blood typing using readily available antibodies.
  • To compare the performance of mixed-clone versus single-clone antibodies in SPR imaging for blood typing.
  • To assess the speed, accuracy, and throughput of SPR imaging compared to standard agglutination tests.

Main Methods:

  • Five antibody groups (mixed and single clones of anti-A, anti-B, anti-AB) were immobilized on a multichannel flow cell for SPR imaging.
  • Red blood cell (RBC) samples were analyzed using the SPR imager with detection line arrays.
  • SPR imaging data was compared with results from standard agglutination tests for 60 blood samples.

Main Results:

  • SPR imaging correctly typed all 60 blood samples in under 12 minutes, consistent with standard agglutination results.
  • Mixed-clone antibodies yielded a 33%-68% greater SPR signal change compared to single-clone antibodies.
  • The SPR imaging technique demonstrated high throughput and accuracy for ABO blood typing.

Conclusions:

  • SPR imaging is a viable, rapid, and accurate method for ABO blood typing using standard antibodies.
  • Mixed-clone antibodies enhance sensitivity in SPR imaging-based blood typing.
  • This approach offers potential for cost reduction, faster turnaround times, and increased throughput in blood typing.