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Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

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Superfluorescent squaraine with efficient two-photon absorption and high photostability.

Kevin D Belfield¹, Mykhailo V Bondar, Hafeez S Haniff

¹Department of Chemistry, University of Central Florida, P.O. Box 162366, Orlando, FL 32816-2366 (USA); CREOL, The College of Optics and Photonics, University of Central Florida, P.O. Box 162366, Orlando, FL 32816-2366 (USA). belfield@ucf.edu.

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry

|September 12, 2013

Summary

We synthesized a novel squaraine derivative with high fluorescence quantum yields and excellent photostability. This new material shows efficient two-photon absorption and superfluorescence, paving the way for advanced photonic applications.

Keywords:

density functional calculations donor-acceptor systems fluorescence hydrogen bonds photochemistry

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Area of Science:

Materials Science
Photophysics
Organic Chemistry

Background:

Squaraine dyes are known for their unique optical properties.
Developing new materials with enhanced photophysical characteristics is crucial for advanced applications.
Understanding structure-property relationships is key to designing novel functional molecules.

Purpose of the Study:

To synthesize and characterize a new symmetrical squaraine derivative.
To investigate its linear photophysical, two-photon absorption (2PA), and superfluorescence properties.
To explore its potential for advanced photonic materials.

Main Methods:

Synthesis of squaraine derivative (1).
Steady-state linear spectral and photochemical property measurements.
Fluorescence lifetime and excitation anisotropy studies.
Open-aperture Z-scan for 2PA spectra.
Femtosecond transient absorption spectroscopy.
Quantum chemical calculations.

Main Results:

High fluorescence quantum yields (≈0.7) and excellent photostability were achieved.
Broadband 2PA spectra with a maximum cross section of ≈1000 GM were obtained.
Efficient superfluorescence was observed.
Femtosecond transient absorption revealed excited-state absorption and gain dynamics.
Quantum chemical simulations supported experimental findings for 1PA and 2PA spectra.

Conclusions:

The synthesized squaraine derivative exhibits promising photophysical properties.
Its characteristics suggest potential for applications in advanced photonic materials.
The good agreement between experimental and simulated spectra aids in predicting material behavior.