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Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation
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Kinetic study on cephamycin C degradation.

Luciana M Brites1, Liliane M Oliveira, Marlei Barboza

  • 1Postgraduate Program in Chemical Engineering, Federal University of São Carlos-UFSCar, São Carlos, SP, Brazil.

Applied Biochemistry and Biotechnology
|September 13, 2013
PubMed
Summary
This summary is machine-generated.

Cephamycin C (CepC) exhibits greater stability than penicillin and clavulanic acid, particularly at neutral pH levels. This antibiotic shows higher degradation rates under very acidic or basic conditions, crucial for optimizing its production.

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Area of Science:

  • Biochemistry
  • Microbiology
  • Pharmaceutical Sciences

Background:

  • Cephamycin C (CepC) is a cephalosporin antibiotic with notable resistance to β-lactamases.
  • β-lactamases are key bacterial enzymes conferring resistance to β-lactam antibiotics.
  • Streptomyces clavuligerus is the producing microorganism for Cephamycin C.

Purpose of the Study:

  • To determine the pH-dependent stability of Cephamycin C.
  • To provide data for optimizing Cephamycin C production, extraction, and purification processes.
  • To compare the stability of CepC with other β-lactam compounds.

Main Methods:

  • Evaluated Cephamycin C stability across a pH range (2.2 to 8.7) at 20 °C.
  • Utilized ultrafiltered broth from S. clavuligerus batch fermentations.
  • Monitored degradation rates over 100 hours.

Main Results:

  • Cephamycin C demonstrated higher stability compared to penicillin and clavulanic acid.
  • Degradation was significantly higher at extreme pH values (2.2 and 8.7).
  • At quasi-neutral pH (6.0-7.6), CepC degradation ranged from 15-20% over 100 hours.

Conclusions:

  • Cephamycin C is relatively stable under quasi-neutral pH conditions.
  • Acidic and basic environments accelerate Cephamycin C degradation.
  • Understanding CepC stability is vital for efficient pharmaceutical development and production.