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[The detection of the surface antigens of Trichomonas vaginalis by using the scanning electron microscope]
The method for the detection of surface antigens, based on antibody aggregation phenomenon, was used in scanning microscopy of T. vaginalis. After Trichomonas treatment with immune blood serum diluted 1:5000--1:10,000 aggregates were detected, consisting of subunits 30-80 nm in diameter, localized on the protozoal cilia, projections, and, less frequently, body. Analysis of the results following the material treatment with colloid gold-conjugated anti-IgG permits a conclusion that the detected aggregates are antibodies to immunogenic surface antigens of T. vaginalis.
The method for the detection of surface antigens, based on antibody aggregation phenomenon, was used in scanning microscopy of T. vaginalis. After Trichomonas treatment with immune blood serum diluted 1:5000--1:10,000 aggregates were detected, consisting of subunits 30-80 nm in diameter, localized on the protozoal cilia, projections, and, less frequently, body. Analysis of the results following the material treatment with colloid gold-conjugated anti-IgG permits a conclusion that the detected aggregates are antibodies to immunogenic surface antigens of T. vaginalis.
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