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Updated: May 7, 2026

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies
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Ex Vivo Corneal Organ Culture Model for Wound Healing Studies

Published on: February 15, 2019

A corneal scarring model.

Daniel J Gibson1, Gregory S Schultz

  • 1Department of Obstetrics and Gynecology, Institute for Wound Research, University of Florida, Gainesville, FL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 14, 2013
PubMed
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Corneal haze develops over five days after injury, driven by cellular processes. A new photographic method objectively quantifies haze, improving the study of anti-haze therapies.

Area of Science:

  • Ophthalmology
  • Corneal disease research
  • Biomedical imaging

Background:

  • Corneal opacification (haze) is a post-injury process.
  • Understanding haze development is crucial for effective therapies.
  • Current quantification methods are subjective and hinder comparative studies.

Purpose of the Study:

  • To investigate the cellular and molecular mechanisms of corneal haze formation.
  • To develop and validate an objective photographic technique for quantifying corneal haze.
  • To improve the assessment of candidate anti-haze therapies.

Main Methods:

  • Utilized an excimer laser to create standardized corneal wounds in rabbit models.
  • Monitored changes in corneal thickness, wound closure (re-epithelialization), and haze development over time.

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Ex Vivo and In Vivo Animal Models for Mechanical and Chemical Injuries of Corneal Epithelium
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Ex Vivo and In Vivo Animal Models for Mechanical and Chemical Injuries of Corneal Epithelium

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Last Updated: May 7, 2026

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies
06:46

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies

Published on: February 15, 2019

Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing
07:28

Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing

Published on: July 10, 2018

Ex Vivo and In Vivo Animal Models for Mechanical and Chemical Injuries of Corneal Epithelium
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Ex Vivo and In Vivo Animal Models for Mechanical and Chemical Injuries of Corneal Epithelium

Published on: April 6, 2022

  • Developed a novel photographic technique for objective haze recording and quantification, supplementing traditional grading systems.

    • Main Results:

    • Established a rabbit corneal scarring model to study haze development.
    • Demonstrated that the new photographic technique provides objective, quantifiable data on haze location and density.
    • Showcased the potential for improved comparison of anti-haze treatment efficacy.

    Conclusions:

    • The developed photographic method offers a significant advancement over subjective grading systems for corneal haze.
    • This objective approach enhances the ability to study corneal haze formation and evaluate therapeutic interventions.
    • The findings facilitate more reliable comparisons between different anti-haze treatment trials.