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Updated: May 7, 2026

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
Published on: June 6, 2025
Production of 6-pentyl-α-pyrone by trichoderma harzianum in solid-state fermentation
Aline de Souza Ramos1, Sorele Batista Fiaux, Selma Gomes Ferreira Leite
1Farmanguinhos, Fundação Oswaldo Cruz , Rio de Janeiro, RJ , Brasil ; Departamento de Engenharia Bioquímica, Escola de Química, Universidade Federal do Rio de Janeiro , Rio de Janeiro, RJ , Brasil.
Abstract:
Many Trichoderma species are able to produce 6-pentyl-α-pyrone (6-PP), a lactone with coconut-like aroma. In the present work, several culture parameters were studied to enhance the production of 6-PP by Trichoderma harzianum 4040 in solid-state fermentation. Green coir powder added to a nutrient solution was used as support material for fermentation. A Plackett-Burman screening technique was applied, followed by a fractionary factorial design. The best culture conditions within the experimental domain studied were (100 g support)(-1): sucrose, 3 g; NaNO3, 0.24 g; (NH4)2SO4, 0.18 g; KH2PO4, 0.1 g; inoculum concentration, 2.2 × 10(6) spores; moisture level, 55%. The temperature established was 28°C. The fermentation under the selected conditions led to a 6-PP production six times higher (5.0 mg/g dry matter) than the initial one (0.8 mg/g dry matter) after seven days of cultivation.
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