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Updated: May 7, 2026
07:57
Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis
Published on: December 8, 2011
Vaginal lactobacilli as potential probiotics against Candida SPP
Natalia F Gil1, Rafael C R Martinez, Bruna C Gomes
1Departmento de Análises Clínicas, Toxicológicas e Bromatológicas,Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo , Ribeirão Preto, SP , Brasil.
Summary
Researchers screened 11 vaginal lactobacilli for probiotic potential against Candida species. Certain isolates, including L. agilis and L. jensenii, showed promising properties for managing vaginal infections.
Area of Science:
- Microbiology
- Probiotics
- Infectious Diseases
Background:
- Urogenital infections are common worldwide, often treated with antimicrobials.
- Probiotic lactic acid bacteria (LAB) are increasingly studied for vaginal infection management.
- Screening for novel probiotic candidates against Candida species is crucial.
Purpose of the Study:
- To evaluate 11 vaginal lactobacilli isolates for probiotic properties against Candida species.
- To assess auto-aggregation, co-aggregation, epithelial cell adhesion, and production of lactic acid and hydrogen peroxide (H2O2).
- To identify potential probiotic strains for vaginal health.
Main Methods:
- Tested 11 vaginal lactobacilli isolates for auto-aggregation and co-aggregation with Candida spp. (C. albicans, C. glabrata, C. krusei, C. tropicalis).
- Assessed adhesion to Caco-2 epithelial cells.
- Quantified lactic acid and hydrogen peroxide (H2O2) production.
Main Results:
- All isolates demonstrated auto-aggregation; L. crispatus showed highest co-aggregation with Candida species.
- L. salivarius produced the most lactic acid (13.9 g/l).
- L. agilis, L. jensenii, L. johnsonii, and L. ruminus adhered to Caco-2 cells and showed potential as probiotic candidates.
Conclusions:
- Several vaginal lactobacilli isolates possess desirable probiotic properties against Candida species.
- L. agilis, L. jensenii, L. johnsonii, and L. ruminus are promising candidates for further development.
- These findings support the use of specific LAB for managing vaginal infections.
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