Related Experiment Video
Updated: May 1, 2026

Bioprospecting of Extremophilic Microorganisms to Address Environmental Pollution
Published on: December 31, 2021
Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp
1Amity Institute of Biotechnology, Amity University , Sector-125, Noida, Uttar Pradesh , India.
Abstract:
Solid state fermentation was carried out using various agro- industrial wastes with the best amylase producing strain isolated from soil. Different physicochemical conditions were varied for maximum enzyme production. The strain produced about 5400 units/g of amylase at 1:3 moisture content, 20% inoculum, after 72 h of incubation with Mustard Oil seed cake as the substrate. The optimum temperature and pH of the enzyme activity were found to be 50°C and 6 respectively. The enzyme was found to be thermostable at 70°C for about 2 h without any salt. It showed stability at pH range 5-7. The metal ions as Na(+), Ca(++), Mg(++) and Co(++) enhanced the enzyme activity.
Related Concept Videos
Microorganisms in Agriculture and Food industry
Microbes in Food Production
Microbes in the Production of Fermented Foods
Production of Organic Acids
Production of Antibiotics
Production of Biopesticides

