Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA as a Genetic Template

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

Dynamic Equilibrium

Dynamic Equilibrium

A reversible chemical reaction represents a chemical process that proceeds in both forward (left to right) and reverse (right to left) directions. When the rates of the forward and reverse reactions are equal, the concentrations of the reactant and product species remain constant over time and the system is at equilibrium. A special double arrow is used to emphasize the reversible nature of the reaction. The relative concentrations of reactants and products in equilibrium systems vary greatly;...

The DNA Helix

The DNA Helix

Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...

The DNA Helix

The DNA Helix

DNA Base Pairing

DNA Base Pairing

Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,

DNA Base Pairing

DNA Base Pairing

Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Investigation Into the Role of Reductants and Cosubstrates in Lytic Polysaccharide Monooxygenase Thermothielavioides terrestris AA9E Binding to Cellulose by Single-Molecule Imaging.

Biotechnology and bioengineering·2025

Same author

Nascent RNA Folding and RNP Assembly Revealed by Single-molecule Microscopy.

Journal of molecular biology·2025

Same author

Super-Resolved Fluorescence Lifetime Imaging of Single Cy3 Molecules and Quantum Dots Using Time-Correlated Single Photon Counting with a Four-Pixel Fiber Optic Array Camera.

The journal of physical chemistry. A·2024

Same author

Click catalysis and DNA conjugation using a nanoscale DNA/silver cluster pair.

Nanoscale·2024

Same author

Breaching the Fortress: Photochemistry of DNA-Caged Ag<sub>10</sub><sup>6</sup>.

The journal of physical chemistry. B·2023

Same author

Kinetic and Thermodynamic Control of G-Quadruplex Polymorphism by Na<sup>+</sup> and K<sup>+</sup> Cations.

The journal of physical chemistry. B·2023

Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026

Same journal

Development of a Tapping-Mode Scanning Probe Electrospray Ionization Platform for High-Sensitivity and Long-Term Stability in Single-Cell Mass Spectrometry Imaging of Tissue.

Analytical chemistry·2026

Same journal

A Solid-State Near-Infrared Fluorescent Probe by a Synergistic Extended Conjugated System for Detecting Cys with Long-Term Imaging in Orthotopic Bladder Cancer.

Analytical chemistry·2026

Same journal

Air-Based Porous Array Dielectric Barrier Discharge Ionization Source for Explosive Trace Detection.

Analytical chemistry·2026

Same journal

PSAQ<sup>+1</sup>: Absolute Protein Quantification Using a <sup>13</sup>C<sub>1</sub>-Labeled Protein Standard, Coisolation of Peptide Pairs and LC-PRM.

Analytical chemistry·2026

Same journal

Heterojunction-Enhanced Interfacial Evanescent-Tunable Fiber Optic Probe for Amplification-free CRISPR/Cas12a-Based Rapid and Ultrasensitive Detection of MPXV.

Analytical chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 7, 2026

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

A silver cluster-DNA equilibrium.

Jeffrey T Petty¹, Orlin O Sergev, David A Nicholson

¹Department of Chemistry, Furman University , Greenville, South Carolina 29613, United States.

Analytical Chemistry

|September 17, 2013

Summary

This summary is machine-generated.

Silver clusters within DNA form molecular sensors. Hybridization triggers a color and emission shift, enabling sensitive detection by modulating DNA structure.

More Related Videos

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Published on: November 1, 2012

Related Experiment Videos

Last Updated: May 7, 2026

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Published on: November 1, 2012

Area of Science:

Nanomaterials Science
Biochemistry
Molecular Sensing

Background:

DNA-templated silver clusters are emerging as sensitive molecular probes.
Understanding the structural and optical transformations of these hybrid nanomaterials is crucial for sensor development.

Purpose of the Study:

To characterize a novel silver cluster-oligonucleotide sensor.
To investigate the structural and spectral changes of silver clusters upon DNA hybridization.

Main Methods:

Synthesis and purification of silver cluster-oligonucleotide conjugates.
Spectroscopic analysis (UV-Vis absorption and fluorescence emission).
Size exclusion chromatography and elemental analysis for structural validation.

Main Results:

A single-stranded DNA template formed a violet silver cluster (λmax = 400 nm) with low emission.
Hybridization with complementary oligonucleotides induced a spectral shift to blue-green (λmax = 490 nm) and developed fluorescence emission at 550 nm.
The silver cluster size (∼11 atoms) remained constant during hybridization, and the transformation was reversible and temperature-dependent.

Conclusions:

Silver cluster-oligonucleotide complexes function as responsive molecular sensors.
The observed spectral changes are directly linked to DNA secondary structure modulation.
These hybrid nanomaterials offer a unique sensing mechanism due to their dual role as reporters and interaction modulators.