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Addition of serum to electroporated cells enhances survival and transfection efficiency
1Department of Radiation Oncology, University of Pittsburgh School of Medicine, PA 15261.
Biochemical and Biophysical Research Communications
|September 14, 1990
Abstract:
Optimal electroporation efficiency of many cell types is associated with poor survival. We show that serum rapidly reseals the membranes of electroporated cells and that timely addition of serum following electroporation can improve cell survival and transfection efficiency.