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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
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Nucleic acids02:43

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
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Related Experiment Video

Updated: May 7, 2026

Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer
06:15

Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer

Published on: February 17, 2011

Explanatory chapter: nucleic acid concentration determination.

Sarah Kolitz1, Jon Lorsch

  • 1Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.

Methods in Enzymology
|September 17, 2013
PubMed
Summary
This summary is machine-generated.

This protocol outlines a method for accurately measuring nucleic acid sample concentrations. Precise quantification is essential for downstream molecular biology applications.

Keywords:
Absorbance value determinationBeer's lawNanoDrop spectrophotometerNucleic acidSpectrophotometersingle-stranded DNA

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Last Updated: May 7, 2026

Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer
06:15

Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer

Published on: February 17, 2011

NanoDrop Microvolume Quantitation of Nucleic Acids
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NanoDrop Microvolume Quantitation of Nucleic Acids

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Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples
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Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

Published on: February 4, 2013

Area of Science:

  • Molecular Biology
  • Biochemistry

Background:

  • Accurate quantification of nucleic acids is critical for reliable downstream applications.
  • Existing methods may have limitations in sensitivity or specificity.

Purpose of the Study:

  • To describe a novel protocol for determining nucleic acid sample concentration.
  • To provide a reliable and reproducible method for nucleic acid quantification.

Main Methods:

  • The protocol involves spectrophotometric analysis.
  • Specific wavelengths are used to measure absorbance.
  • Concentration is calculated based on absorbance values.

Main Results:

  • The method demonstrated high accuracy in determining nucleic acid concentrations.
  • Reproducibility was confirmed across multiple trials.
  • The protocol is adaptable to various nucleic acid types.

Conclusions:

  • This protocol offers a robust method for nucleic acid concentration determination.
  • It is suitable for diverse research and diagnostic applications.
  • Accurate nucleic acid quantification is fundamental for molecular biology research.