Related Experiment Video
Updated: May 7, 2026

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
Published on: April 16, 2018
Direct electron transfer from electrode to electrochemically active bacteria in a bioelectrochemical dechlorination
Ding Liu1, Lecheng Lei, Bin Yang
1Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Yuquan Campus, Zhejiang University, Hangzhou, Zhejiang 310027, China.
Abstract:
Pentachlorophenol (PCP) was dechlorinated by electrochemically active bacteria using an electrode as the direct electron donor. Dechlorination efficiency and coulombic efficiency (CE) were investigated. When hydrogen evolution reaction was eliminated by controlling the potential, both dechlorination efficiency and CE increase as the potential decreases, which implied the dechlorination was stimulated by electric current rather than hydrogen gas. Further investigation of the cyclic voltammetry characterization of the medium revealed nearly no redox mediator secreted by the bacteria. Moreover, the comparison of dechlorination experiments carried out with filtered and unfiltered medium provided convincible evidence that the dominating electron transfer mechanism for the dechlorination is direct electron transfer. Additionally, 454 pyrosequencing technique was employed to gain a comprehensive understanding of the biocathodic microbial community. The results showed Proteobacteria, Bacteroidetes and Firmicutes were the three predominant groups. This paper demonstrated the direct electron transfer mechanism could be involved in PCP dechlorination with a biocathode.
More Related Videos
Related Concept Videos
Metabolism of Chemolithotrophs
Microbial Bioremediation of Pesticides
Electrochemical Cells
Microbial Leaching
Bioremediation
Anoxygenic Photosynthesis

