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Effects of methotrexate on transfected DNA stability in mammalian cells
M G Pallavicini1, P S DeTeresa, C Rosette
1Biomedical Sciences Division, Lawrence Livermore National Laboratory, Livermore, California 94550.
Molecular and Cellular Biology
|January 1, 1990
Abstract:
The chromosomal locations, amounts, and level of expression of transfected, amplified c-myc and dihydrofolate reductase sequences were measured in cells cultured in the presence and absence of methotrexate. These studies show that the location and amount of transfected sequences, as well as the level of expression, were more variable when the cells were cultured in methotrexate.