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Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

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Published on: February 23, 2016

Formulation and utilization of choline based samples for dissolution dynamic nuclear polarization.

Sean Bowen¹, Jan Henrik Ardenkjaer-Larsen

¹Technical University of Denmark, Department of Electrical Engineering, Kgs. Lyngby, Denmark.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)

|September 17, 2013

Summary

This summary is machine-generated.

Dissolution dynamic nuclear polarization (DNP) enables high spin polarization. Choline-based formulations offer stable, highly soluble samples for improved DNP performance and easier post-experiment processing.

Keywords:

Dynamic nuclear polarization Glassing agents Hyperpolarization NMR

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Area of Science:

Nuclear Magnetic Resonance (NMR) Spectroscopy
Chemical Engineering
Biophysics

Background:

Dissolution dynamic nuclear polarization (DNP) is a technique for enhancing NMR signal sensitivity.
Sample formulation for dissolution-DNP is challenging due to the need to balance concentration, viscosity, and polarization stability.
Traditional formulations often require complex optimization for successful dissolution-DNP experiments.

Purpose of the Study:

To explore choline and its derivatives as novel agents for dissolution-DNP sample formulation.
To compare the performance of choline-based formulations with traditional DNP sample matrices.
To assess the advantages of choline in terms of solubility, stability, and post-experiment processing.

Main Methods:

Formulation of choline-based salts and deep eutectic mixtures with carboxylic acids and urea.
Preparation and characterization of dissolution-DNP samples using choline-based matrices.
Comparison of polarization levels and sample stability against conventional DNP formulations.
Evaluation of choline removal and potential for experiments with hyperpolarized choline.

Main Results:

Choline enables the creation of highly soluble salts and deep eutectic mixtures suitable for dissolution-DNP.
Choline-based samples demonstrate exceptional polarization performance and stability during dissolution.
These formulations offer simpler handling and versatile options for post-experiment analysis, including the use of hyperpolarized choline.

Conclusions:

Choline is a highly effective component for developing robust and high-performance dissolution-DNP samples.
Choline-based formulations overcome key challenges in sample preparation for dissolution-DNP.
The use of choline provides significant advantages in terms of experimental flexibility and efficiency in DNP-NMR studies.