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Updated: May 7, 2026
Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
Published on: January 21, 2020
Development of animal experimental periodontitis models
Min-Jae Do1, Kyuri Kim, Haeshin Lee
1Department of Chemistry, Graduate School of Nanoscience and Technology (WCU), Korea Advanced Institute of Science and Technology, Seoul, Korea. ; Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry, Seoul, Korea.
Introducing lipopolysaccharide (LPS) from Porphyromonas gingivalis to alveolar bone defects in dogs created a viable experimental periodontitis model. This method effectively induced periodontal inflammation for further research.
Area of Science:
- Periodontology
- Veterinary Pathology
- Oral Microbiology
Background:
- Developing a reliable animal model for periodontitis is crucial for understanding disease mechanisms and testing treatments.
- Lipopolysaccharide (LPS) from periodontal pathogens plays a key role in initiating and exacerbating periodontal disease.
Purpose of the Study:
- To evaluate the suitability of using Porphyromonas gingivalis LPS (P.g. LPS) in surgically created alveolar bone defects as a model for experimental periodontitis.
- To assess the inflammatory response and disease progression in this proposed periodontitis model.
Main Methods:
- Alveolar bone defects were surgically created in beagle dogs.
- Experimental groups included silk ligature, P.g. LPS-saturated collagen with silk ligature, and a control group.
- Plaque and gingival indices were measured, followed by histologic evaluation of tissues.
Main Results:
- Both the silk ligature and P.g. LPS groups showed increased plaque indices compared to controls.
- The P.g. LPS group exhibited a significantly higher gingival index, indicating greater inflammation.
- Histologic analysis confirmed increased inflammatory cell infiltration in the gingival tissue and alveolar bone of the P.g. LPS group.
Conclusions:
- The P.g. LPS-saturated collagen with silk ligature effectively induced periodontal inflammation over 4 weeks.
- This P.g. LPS application to alveolar bone defects represents a promising candidate model for experimental periodontitis research.
