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Updated: May 7, 2026

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
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Published on: February 15, 2021

Stem cell antigen-1 in skeletal muscle function.

Harold S Bernstein1, Tahmina Samad, Sompob Cholsiripunlert

  • 1University of California San Francisco, San Francisco, California, USA.

Plos Currents
|September 18, 2013
PubMed
Summary
This summary is machine-generated.

Stem cell antigen-1 (Sca-1) is crucial for muscle conditioning and exercise adaptation. Sca-1 deficient mice show reduced stamina and impaired muscle relaxation, especially with aging.

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Published on: April 8, 2014

Area of Science:

  • Muscle stem cell biology
  • Exercise physiology
  • Aging research

Background:

  • Stem cell antigen-1 (Sca-1) is a membrane protein found on muscle stem cells and precursors.
  • Previous studies indicated Sca-1 inhibition enhances myoblast proliferation and muscle repair.
  • The role of Sca-1 in normal postnatal muscle function and adaptation remains unclear.

Purpose of the Study:

  • To investigate the function of Sca-1 in normal postnatal muscle.
  • To compare young and aging Sca-1 knockout (KO) and wild-type (WT) mice.
  • To elucidate the effects of Sca-1 deficiency on muscle tissue, contractility, and function.

Main Methods:

  • Systematic comparison of Sca-1-/- (KO) and Sca-1+/+ (WT) mice.
  • Analysis of hindlimb muscle characteristics (volume, fibrosis, myofiber size, myoblast number).
  • Exercise protocols to assess stamina and conditioning; myomechanical analysis of isolated muscles.

Main Results:

  • No significant differences in muscle volume, fibrosis, myofiber size, or myoblast number between genotypes or ages.
  • KO mice exhibited decreased exercise stamina compared to WT mice, with young KO mice performing like aged WT mice.
  • KO mice showed impaired conditioning with exercise and incomplete muscle relaxation upon fatigue, despite generating more force initially.

Conclusions:

  • Sca-1 is essential for normal muscle conditioning and adaptation to exercise.
  • Sca-1 deficiency leads to reduced stamina and impaired muscle relaxation, with age exacerbating these deficits.
  • Sca-1 plays a critical role in maintaining muscle function and exercise capacity throughout the lifespan.