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Updated: May 7, 2026

Visualizing the Developing Brain in Living Zebrafish using Brainbow and Time-lapse Confocal Imaging

Visualizing the Developing Brain in Living Zebrafish using Brainbow and Time-lapse Confocal Imaging

Published on: March 23, 2020

Anatomical dissection of zebrafish brain development.

Katherine J Turner¹, Thomas G Bracewell, Thomas A Hawkins

¹Department of Cell and Developmental Biology, University College London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)

|September 20, 2013

Summary

This resource provides an online neuroanatomical atlas of the embryonic zebrafish brain using high-resolution imaging. It details the materials and protocols used to generate comprehensive data for understanding zebrafish brain development.

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Area of Science:

Neuroscience
Developmental Biology
Zebrafish Model Systems

Background:

The zebrafish (Danio rerio) is a widely used model organism in developmental biology and neuroscience.
A comprehensive neuroanatomical atlas is crucial for studying embryonic brain development and function.
Existing atlases may lack detailed, high-resolution imaging of early developmental stages.

Purpose of the Study:

To create a detailed online neuroanatomical atlas of the embryonic zebrafish brain.
To provide high-resolution confocal images and movies of transgenic zebrafish lines.
To document the materials and protocols used for data generation.

Main Methods:

Utilizing high-resolution confocal microscopy.
Employing transgenic zebrafish lines for specific cell labeling.

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Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy

Published on: April 1, 2009

Dissection of Organs from the Adult Zebrafish

Dissection of Organs from the Adult Zebrafish

Published on: March 4, 2010

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Last Updated: May 7, 2026

Visualizing the Developing Brain in Living Zebrafish using Brainbow and Time-lapse Confocal Imaging

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Published on: March 23, 2020

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy

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Published on: April 1, 2009

Dissection of Organs from the Adult Zebrafish

Dissection of Organs from the Adult Zebrafish

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Developing and refining protocols for tissue preparation and imaging.

Compiling data into an accessible online atlas format.

Main Results:

Generation of a comprehensive digital atlas of the embryonic zebrafish brain.
Provision of detailed visual data on various brain structures.
Establishment of reproducible methods for atlas data acquisition.

Conclusions:

The Zebrafishbrain.org atlas serves as a valuable resource for researchers studying zebrafish neurodevelopment.
The detailed imaging and protocols facilitate comparative studies and further research.
This atlas enhances the utility of zebrafish as a model for brain research.