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Related Experiment Video

Updated: May 7, 2026

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
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In utero electroporation to study mouse brain development.

Emilie Pacary1, François Guillemot

  • 1Neurocentre Magendie, Physiopathologie de la Plasticité Neuronale, U862, Institut National de la Santé et de la Recherche Médicale, Université de Bordeaux, Bordeaux, France.

Methods in Molecular Biology (Clifton, N.J.)
|September 20, 2013
PubMed
Summary
This summary is machine-generated.

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In utero electroporation is a powerful in vivo method for studying brain development. This technique offers advantages for examining processes from cell proliferation to synaptic integration across various brain regions.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Molecular Biology

Background:

  • Studying brain development in vivo is crucial for understanding neurological disorders.
  • Existing methods have limitations in targeting specific developmental stages or brain regions.

Purpose of the Study:

  • To provide a detailed protocol for in utero electroporation.
  • To highlight the versatility of this technique for studying diverse aspects of brain development.

Main Methods:

  • Detailed step-by-step description of in utero electroporation.
  • Demonstration of variations for targeting different brain structures.
  • Application for studying proliferation and synaptic integration.

Main Results:

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In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos
26:17

In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos

Published on: July 19, 2007

Related Experiment Videos

Last Updated: May 7, 2026

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
09:30

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

Published on: August 15, 2011

Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations
10:45

Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations

Published on: June 14, 2020

In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos
26:17

In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos

Published on: July 19, 2007

  • Successful implementation of in utero electroporation across multiple brain regions.
  • Demonstrated ability to track cell development from proliferation to integration.
  • Adaptability of the technique for specific research questions.

Conclusions:

  • In utero electroporation is an effective and adaptable technique for in vivo brain development research.
  • This method facilitates detailed study of cellular processes from proliferation to synaptic integration.
  • The described protocol and variations enable broad application in neuroscience research.