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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: May 7, 2026

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs

Published on: February 2, 2024

Label-free high-throughput cell screening in flow.

Ata Mahjoubfar¹, Claire Chen, Kayvan R Niazi

¹Department of Electrical Engineering, University of California, Los Angeles, California 90095 USA ; California NanoSystems Institute, Los Angeles, California 90095 USA.

Biomedical Optics Express

|September 20, 2013

Summary

This summary is machine-generated.

We developed a label-free flow cytometer that measures cell size and protein concentration simultaneously. This method enhances cell classification accuracy without needing fluorescent labels, improving cell analysis in biotechnology and medicine.

Keywords:

(100.5070) Phase retrieval (170.0180) Microscopy (170.1530) Cell analysis (170.3890) Medical optics instrumentation (170.7160) Ultrafast technology

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Published on: September 28, 2016

Area of Science:

Biotechnology
Medical diagnostics
Cellular analysis

Background:

Flow cytometry is crucial for cell counting and biomarker detection, particularly in blood analysis.
Conventional methods rely on fluorescent cell surface labeling, which can negatively impact cell viability and behavior.
Existing techniques for cell classification estimate size and granularity using scattered light and fluorescence emission spectra.

Purpose of the Study:

To introduce a novel label-free imaging-based flow cytometer.
To enable simultaneous measurement of cell size and protein concentration.
To improve cell classification specificity and sensitivity without cell labeling.

Main Methods:

Development of a label-free imaging-based flow cytometer.
Simultaneous measurement of cell size and protein concentration.
Utilizing coherent dispersive Fourier transform for phase imaging at high flow speeds (meters per second).

Main Results:

The new system measures cell size and protein concentration simultaneously, offering a label-free approach.
Cell protein concentration serves as an additional parameter for improved cell classification.
The technology functions as a stand-alone instrument or an add-on to conventional flow cytometers.

Conclusions:

Label-free flow cytometry using cell protein concentration enhances specificity and sensitivity.
This technique avoids the drawbacks of cell surface labeling, preserving cellular function.
The developed imaging-based flow cytometer offers a promising advancement for cell analysis in various scientific fields.