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Related Experiment Videos

Human bone sialoprotein. Deduced protein sequence and chromosomal localization.

L W Fisher1, O W McBride, J D Termine

  • 1Bone Research Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.

The Journal of Biological Chemistry
|February 5, 1990
PubMed
Summary

Researchers identified the human bone sialoprotein gene on chromosome 4. This protein is crucial for bone cell function and has conserved regions with rat bone sialoprotein, including cell attachment sites.

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Bone sialoprotein is a key non-collagenous matrix protein in bone.
  • Understanding its genetic basis and sequence is vital for bone biology research.

Purpose of the Study:

  • To isolate and characterize the cDNA encoding human bone sialoprotein.
  • To determine the protein sequence and identify conserved functional domains.
  • To map the human bone sialoprotein gene to a specific chromosome.

Main Methods:

  • cDNA library screening using radiolabeled rat bone sialoprotein cDNA.
  • DNA sequencing of the isolated clone.
  • Northern and Southern hybridization analyses.
  • Somatic cell hybrid mapping.

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Main Results:

  • Isolated a 3-kilobase cDNA clone encoding a 317-amino acid human bone sialoprotein.
  • Deduced sequence shows >70% identity with rat bone sialoprotein, including the Arg-Gly-Asp (RGD) cell attachment motif.
  • Identified conserved acidic and tyrosine-rich regions, with potential for tyrosine sulfation.
  • Gene localized to human chromosome 4; confirmed as a single-copy gene.
  • Bone cells are the primary source of bone sialoprotein mRNA.

Conclusions:

  • The human bone sialoprotein gene is located on chromosome 4.
  • The deduced protein sequence provides insights into its function, post-translational modifications, and evolutionary conservation.
  • Human bone sialoprotein lacks cystine residues and is heavily glycosylated.