Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

7.1K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.1K
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

2.9K
Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
2.9K
The Extracellular Matrix01:29

The Extracellular Matrix

10.7K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
10.7K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

2.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.7K
Introduction to Fibroblasts01:09

Introduction to Fibroblasts

3.9K
Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
3.9K
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

4.9K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
4.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discoidin Domain Receptor 2 Is Required for Tooth Extraction Socket Healing.

Journal of dental research·2026
Same author

Dentoalveolar defects and impaired alveolar bone healing in a neural crest directed conditional knockout mouse model of hypophosphatasia.

Bone·2025
Same author

Exogenous bone sialoprotein improves extraction socket healing in Ibsp knockout and wild-type mice.

Bone·2024
Same author

The role of bone sialoprotein in bone healing.

Journal of structural biology·2024
Same author

Murine IRF8 Mutation Offers New Insight into Osteoclast and Root Resorption.

Journal of dental research·2024
Same author

Bone Sialoprotein Is Critical for Alveolar Bone Healing in Mice.

Journal of dental research·2022

Related Experiment Video

Updated: Apr 27, 2026

Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay
11:38

Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay

Published on: September 14, 2021

2.4K

Fibromodulin and Biglycan Modulate Periodontium through TGFβ/BMP Signaling.

L Wang1, B L Foster2, V Kram3

  • 1National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS), National Institutes of Health (NIH), Bethesda, MD, USA le.wang@nih.gov.

Journal of Dental Research
|June 27, 2014
PubMed
Summary
This summary is machine-generated.

Small leucine-rich proteoglycans (SLRPs) are crucial for maintaining periodontal homeostasis. Their deficiency disrupts collagen organization and bone remodeling, impacting periodontal ligament function and signaling pathways.

Keywords:
extracellular matrixmineralizationperiodontal ligamentproteoglycansignal transductiontooth root

More Related Videos

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
06:54

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

13.1K
Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

4.2K

Related Experiment Videos

Last Updated: Apr 27, 2026

Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay
11:38

Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay

Published on: September 14, 2021

2.4K
Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
06:54

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

13.1K
Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

4.2K

Area of Science:

  • Biochemistry
  • Extracellular Matrix Biology
  • Periodontal Research

Background:

  • Small leucine-rich proteoglycans (SLRPs) are extracellular matrix components involved in collagen organization and cell signaling.
  • The specific roles of SLRPs, such as fibromodulin and biglycan, in periodontal development and homeostasis are not well understood.
  • Understanding these regulators is vital for advancing periodontal regenerative therapies.

Purpose of the Study:

  • To investigate the role of fibromodulin and biglycan deficiency in the periodontium.
  • To elucidate the effects of SLRPs on periodontal ligament (PDL) structure, alveolar bone remodeling, and signaling pathways.

Main Methods:

  • Transmission electron microscopy (TEM) to analyze collagen fibril structure.
  • Immunohistochemistry (IHC) to assess the expression of various proteins including SLRPs, DMP1, and signaling molecules.
  • Polymerase chain reaction (PCR) array to evaluate gene expression related to TGFβ/BMP signaling.

Main Results:

  • Mice lacking fibromodulin and biglycan (dKO) exhibited abnormal collagen fibrils in the PDL and altered alveolar bone remodeling.
  • Increased expression of certain SLRPs and DMP1 was observed, while osteoblast markers remained unchanged.
  • dKO mice showed increased RANKL expression, elevated osteoclast numbers, and hyperactive TGFβ/BMP signaling.

Conclusions:

  • SLRPs are essential for maintaining periodontal homeostasis.
  • SLRPs regulate collagen organization, matrix turnover, and TGFβ/BMP signaling in the periodontium.
  • Deficiency in specific SLRPs leads to disruptions in periodontal structure and function, offering insights for regenerative therapies.