Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration01:28

Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration

174
Glomerular filtration rate (GFR) can be estimated from serum creatinine using the modification of diet in renal disease (MDRD) formula or the chronic kidney disease–epidemiology collaboration (CKD–EPI) equation. Both methods are widely used in clinical practice to assess kidney function and guide treatment decisions.The MDRD equation does not require weight or height measurements and is normalized to the body surface area of 1.73 m², considered the average adult surface area.
174
Drug Dosing in Renal Diseases: Measurement of Serum Creatinine Concentration and Clearance01:25

Drug Dosing in Renal Diseases: Measurement of Serum Creatinine Concentration and Clearance

171
In healthy individuals, serum creatinine levels remain stable due to a balance between its constant production—primarily from muscle metabolism—and renal excretion. Creatinine is freely filtered by the glomeruli, making it a valuable marker for estimating renal function. When the glomerular filtration rate (GFR) decreases, the kidneys can only eliminate less creatinine, causing serum levels to rise.Serum creatinine concentration is widely used to estimate creatinine clearance...
171
Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

601
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
601
Renal Drug Clearance: Comparison Between Renal Excretion Methods01:08

Renal Drug Clearance: Comparison Between Renal Excretion Methods

537
Renal clearance is a critical parameter encompassing kidney filtration, secretion, and reabsorption processes. It is calculated using a specific equation to determine the rate at which the kidneys clear a drug.
Renal clearance is often associated with the renal glomerular filtration rate (GFR), which represents the rate at which plasma is filtered through the glomeruli in the kidney. When drug reabsorption is minimal and there is no active secretion, renal clearance is closely related to the...
537
Determination of Renal Drug Clearance: Graphical and Midpoint Methods01:07

Determination of Renal Drug Clearance: Graphical and Midpoint Methods

361
Renal clearance, a crucial parameter in pharmacokinetics, can be determined using two different methods: the graphical method and the midpoint method. These methods provide insights into the rate of drug excretion by the kidneys and aid in assessing renal function.
The graphical method involves plotting the rate of drug excretion in urine against the plasma drug concentration. By analyzing the graph, the clearance can be calculated and obtained. Drugs rapidly excreted by the kidneys exhibit a...
361
EDTA: Indirect and Alkalimetric Titration01:23

EDTA: Indirect and Alkalimetric Titration

1.7K
Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Forensic Electrochemistry: A Dual-Mode Strategy for Rapid and Selective Detection of Catecholamine Compounds.

Analytical chemistry·2026
Same author

Advances in Additive Manufacturing Electrochemistry.

Chemical reviews·2026
Same author

Local Coordination Environment Engineering of Na3 Sites in Na<sub>4</sub>Mn<sub>1.5</sub>Fe<sub>1.5</sub>(PO<sub>4</sub>)<sub>2</sub>P<sub>2</sub>O<sub>7</sub> Cathode.

Journal of the American Chemical Society·2026
Same author

Accuracy of marginal and internal fit of additively manufactured single unit crowns: A systematic review and meta-analysis.

The Journal of prosthetic dentistry·2026
Same author

Hydrophobic Deep Eutectic Solvent-Enhanced Filaments: A Green Breakthrough for Additive-Manufactured Electrodes.

ChemSusChem·2026
Same author

Automated air plasma-assisted functionalization of graphite electrodes for enhanced electrochemical sensing of uric acid.

Mikrochimica acta·2026

Related Experiment Video

Updated: Jan 10, 2026

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging
04:33

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

1.4K

An improved electrochemical creatinine detection method via a Jaffe-based procedure.

Edward P Randviir1, Dimitrios K Kampouris, Craig E Banks

  • 1Faculty of Science and Engineering, School of Chemistry and the Environment, Division of Chemistry and Environmental Science, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, Lancs, UK. c.banks@mmu.ac.uk.

The Analyst
|September 21, 2013
PubMed
Summary

An enzymeless electrochemical method detects creatinine by measuring consumed picrate anions. This method shows promise for point-of-care diagnostics, especially for assessing kidney function through urinary creatinine levels.

More Related Videos

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
09:32

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

11.4K
Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney
10:00

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney

Published on: October 12, 2015

12.2K

Related Experiment Videos

Last Updated: Jan 10, 2026

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging
04:33

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

1.4K
Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
09:32

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

11.4K
Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney
10:00

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney

Published on: October 12, 2015

12.2K

Area of Science:

  • Electrochemistry
  • Analytical Chemistry
  • Biomedical Diagnostics

Background:

  • Creatinine detection is crucial for assessing kidney function.
  • Existing methods can be complex or time-consuming.
  • Development of rapid, sensitive, and cost-effective detection methods is needed.

Purpose of the Study:

  • To develop and validate an enzymeless electrochemical method for creatinine detection.
  • To assess the method's applicability for measuring urinary creatinine.
  • To evaluate the potential for point-of-care application.

Main Methods:

  • Indirect electrochemical detection of picrate anion consumption upon reaction with creatinine.
  • Utilized Edge Plane Pyrolytic Graphite (EPPG) and screen-printed carbon electrodes.
  • Optimized reaction conditions (pH 13) and reaction time (5 minutes).

Main Results:

  • Two linear analytical ranges were identified for EPPG electrodes (0-6 mM and 7.5-11.5 mM) with a limit of detection of 0.27 mM.
  • Screen-printed carbon electrodes showed similar ranges (0-6 mM and 6-11 mM) with a limit of detection of 0.72 mM.
  • The method successfully detected urinary creatinine in patient samples, indicating normal kidney function and aligning with UV/Vis spectrometry benchmarks.

Conclusions:

  • The developed electrochemical protocol offers a sensitive and reliable method for creatinine detection.
  • The use of screen-printed electrodes supports potential for cost-effective, portable diagnostic devices.
  • This technology could be applied as a point-of-care system for kidney function assessment, particularly for urinary creatinine levels in various patient populations.