Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterizing the effects of MSC lipid remodeling via sphingomyelinase licensing through morphological, metabolic, and secretome profiling.

Cytotherapy·2026
Same author

Investigating FTY720-NF immunomodulation of key lipid, gene, and protein mediators to enhance oral tissue regeneration.

Biomaterials advances·2026
Same author

PAVR: High-Resolution Cellular Imaging via a Physics-Aware Volumetric Reconstruction Framework.

bioRxiv : the preprint server for biology·2026
Same author

Exogenous sphingomyelinase mediates MSC-derived EV biogenesis and enhances potency via repackaging of molecular cargo.

iScience·2026
Same author

Radiomic Features Extracted from Magnetic Resonance Imaging to Identify Arteriopathy in a Humanized Mouse Model of Sickle Cell Anemia and Sickle Cell Trait.

Annals of biomedical engineering·2026
Same author

Resolving versus non-resolving sphingolipid dynamics during macrophage activation: a time-resolved metabolic analysis.

Journal of lipid research·2025

Related Experiment Video

Updated: May 7, 2026

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size
13:46

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size

Published on: October 17, 2016

Multilayer cell-seeded polymer nanofiber constructs for soft-tissue reconstruction.

Daniel A Barker1, Daniel T Bowers, Brian Hughley

  • 1Department of Otolaryngology-Head & Neck Surgery, University of Virginia, Charlottesville.

JAMA Otolaryngology-- Head & Neck Surgery
|September 21, 2013
PubMed
Summary
This summary is machine-generated.

Cell seeding in nanofiber scaffolds enhances vascularization for tissue engineering. This technique offers a promising, patient-specific alternative to traditional implants, improving integration and reducing inflammation.

More Related Videos

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Electrospun Fibrous Scaffolds of Poly(glycerol-dodecanedioate) for Engineering Neural Tissues From Mouse Embryonic Stem Cells
08:03

Electrospun Fibrous Scaffolds of Poly(glycerol-dodecanedioate) for Engineering Neural Tissues From Mouse Embryonic Stem Cells

Published on: June 18, 2014

Related Experiment Videos

Last Updated: May 7, 2026

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size
13:46

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size

Published on: October 17, 2016

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Electrospun Fibrous Scaffolds of Poly(glycerol-dodecanedioate) for Engineering Neural Tissues From Mouse Embryonic Stem Cells
08:03

Electrospun Fibrous Scaffolds of Poly(glycerol-dodecanedioate) for Engineering Neural Tissues From Mouse Embryonic Stem Cells

Published on: June 18, 2014

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Nanofiber scaffolds offer potential for patient-specific implants, but the impact of cell seeding on thick constructs remains understudied.
  • Traditional implants can cause inflammation and have limited integration.
  • Cell seeding may enhance implant integration and long-term efficacy.

Purpose of the Study:

  • To develop and evaluate a novel cell-preseeded nanofiber tissue engineering technique.
  • To create a 3D biocompatible implant alternative to decellularized extracellular matrix.
  • To investigate the effects of cell seeding on thick nanofiber constructs in vivo.

Main Methods:

  • An animal study using Sprague-Dawley rats (n=36) was conducted.
  • Four implant types were tested: cell-seeded PCL/collagen nanofiber scaffolds, cell-free PCL/collagen nanofiber scaffolds, AlloDerm, and ENDURAGen.
  • Scaffold biocompatibility, soft-tissue remodeling, vascularization, and cell distribution were assessed using histology, micro-computed tomography, and immunohistochemistry.

Main Results:

  • Fibroblasts successfully migrated and remained viable within nanofiber layers for up to 14 days.
  • Cell-seeded scaffolds exhibited minimal inflammation and promoted rapid vascularization.
  • Implant volume was not significantly affected by cell preseeding up to 8 weeks, and donor cell presence declined over time.

Conclusions:

  • Polymer nanofiber scaffolds mimic the natural extracellular matrix and are biocompatible.
  • Cell preseeding enhances vascularization in nanofiber constructs, independent of donor cell persistence.
  • This 3D cell-seeding technique is feasible for clinical application, potentially impacting reconstructive surgery.