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Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging
Published on: September 17, 2009
Confocal microscopy of cardiac myocytes
Robert L Price1, Stephen T Haley, Tara Bullard
1Department of Cell and Developmental Biology and Anatomy, School of Medicine, University of South Carolina, Columbia, SC, USA.
Methods in Molecular Biology (Clifton, N.J.)
|September 21, 2013
Abstract:
Detailed methods are provided for the preparation and confocal imaging of cardiac myocyte development and differentiation. Examples include protocols for the analysis of cultured myocytes as well as vibratome sections of hearts from embryonic and adult tissue. Techniques include routine labeling of F-actin with phalloidin as well as multiple labeling protocols for colocalization studies and cell volume analysis.
